Yoshikawa Masayuki, Wang Tao, Sugimoto Sachiko, Nakamura Seikou, Nagatomo Akifumi, Matsuda Hisashi, Harima Shoichi
Kyoto Pharmaceutical University, shityono-cho, Misasagi, Yamashina-ku, Kyoto, Japan.
Yakugaku Zasshi. 2008 Jan;128(1):141-51. doi: 10.1248/yakushi.128.141.
As a part of our characterization studies on the bioactive saponin constituents of tea flowers (Camellia sinensis, flower buds), the methanolic extract and 1-butanol-soluble portion (the saponin fraction) from the flower buds were found to exhibit potent inhibitory effects on ethanol- and indomethacin-induced gastric mucosal lesions in rats and on serum glucose elevation in sucrose-loaded rats. Among the constituents of the 1-butanol-soluble portion, floratheasaponins A, B, and C showed gastroprotective and hypoglycemic activities. Furthermore, we have developed qualitative and quantitative methods using HPLC for the principle saponins, floratheasaponins A-F, in tea flowers, which were previously found to show antiallergic and antiobesity effects. Using those methods, the saponin composition of Indian tea flowers were found to be similar to those of Chinese (Anhui) but not of Japanese tea flowers. On the other hand, it was found that the floratheasaponin contents in tea flowers varied markedly during the blooming period, and they were abundant at half-bloom. Additionally, the contents of caffeine in the tea flowers were examined using HPLC.
作为我们对茶花（茶树，花芽）生物活性皂苷成分表征研究的一部分，发现花芽的甲醇提取物和1-丁醇可溶部分（皂苷部分）对大鼠乙醇和吲哚美辛诱导的胃黏膜损伤以及蔗糖负荷大鼠的血糖升高具有显著的抑制作用。在1-丁醇可溶部分的成分中，茶花花皂苷A、B和C表现出胃保护和降血糖活性。此外，我们开发了使用高效液相色谱法对茶花中主要皂苷茶花花皂苷A-F进行定性和定量的方法，这些皂苷先前被发现具有抗过敏和抗肥胖作用。使用这些方法，发现印度茶花的皂苷组成与中国（安徽）茶花相似，但与日本茶花不同。另一方面，发现茶花中的茶花花皂苷含量在开花期有显著变化，在半开时含量丰富。此外，使用高效液相色谱法检测了茶花中的咖啡因含量。
