瞬时受体电位香草酸亚型3（TRPC3）通道转录本与促炎细胞因子的关联。

Association of transient receptor potential canonical type 3 (TRPC3) channel transcripts with proinflammatory cytokines.

作者信息

Thilo Florian, Scholze Alexandra, Liu Dao Yan, Zidek Walter, Tepel Martin

机构信息

Charité Campus Benjamin Franklin, Med. Klinik Nephrologie, Hindenburgdamm 30, 12200 Berlin, Germany.

出版信息

Arch Biochem Biophys. 2008 Mar 1;471(1):57-62. doi: 10.1016/j.abb.2007.12.006. Epub 2007 Dec 23.

DOI:10.1016/j.abb.2007.12.006

PMID:18177730

Abstract

We investigated whether expression of non-selective cation channels of the transient receptor potential canonical (TRPC) channel family are associated with proinflammatory cytokines in monocytes. Using quantitative RT-PCR we studied the expression of TRPC3, interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) in monocytes from 15 patients with essential hypertension and 16 age- and sex-matched normotensive control subjects. We observed an approximately 8-fold increase of TRPC3 transcripts in monocytes from patients with essential hypertension compared to normotensive control subjects (p<0.05). We found an approximately 3-fold increase of IL-1beta, and an approximately 9-fold increase of TNF-alpha in patients with essential hypertension compared to normotensive control subjects (each p<0.05). We observed a significant correlation between TRPC3 transcripts with systolic blood pressure, expression of IL-1beta, and TNF-alpha. Using quantitative RT-PCR we observed an association of TRPC3 transcripts and proinflammatory cytokines in monocytes.

摘要

我们研究了瞬时受体电位香草酸亚型（TRPC）通道家族的非选择性阳离子通道的表达是否与单核细胞中的促炎细胞因子相关。我们使用定量逆转录聚合酶链反应（RT-PCR）研究了15例原发性高血压患者及16例年龄和性别匹配的血压正常对照者单核细胞中TRPC3、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的表达。我们观察到，与血压正常的对照者相比，原发性高血压患者单核细胞中TRPC3转录本增加了约8倍（p<0.05）。我们发现，与血压正常的对照者相比，原发性高血压患者的IL-1β增加了约3倍，TNF-α增加了约9倍（均p<0.05）。我们观察到TRPC3转录本与收缩压、IL-1β表达及TNF-α之间存在显著相关性。通过定量RT-PCR，我们观察到单核细胞中TRPC3转录本与促炎细胞因子之间存在关联。