Kampf Günter, Hollingsworth Angela
Bode Chemie GmbH & Co, KG, Scientific Affairs, Melanchthonstr, 27, 22525 Hamburg, Germany.
Ann Clin Microbiol Antimicrob. 2008 Jan 22;7:2. doi: 10.1186/1476-0711-7-2.
Some studies indicate that the commonly recommended 30 s application time for the post contamination treatment of hands may not be necessary as the same effect may be achieved with some formulations in a shorter application time such as 15 s.
We evaluated the bactericidal activity of an ethanol-based hand gel (Sterillium Comfort Gel) within 15 s in a time-kill-test against 11 Gram-positive, 16 Gram-negative bacteria and 11 emerging bacterial pathogens. Each strain was evaluated in quadruplicate.
The hand gel (85% ethanol, w/w) was found to reduce all 11 Gram-positive and all 16 Gram-negative bacteria by more than 5 log10 steps within 15 s, not only against the ATCC test strains but also against corresponding clinical isolates. In addition, a log10 reduction > 5 was observed against all tested emerging bacterial pathogens.
The ethanol-based hand gel was found to have a broad spectrum of bactericidal activity in only 15 s which includes the most common species causing nosocomial infections and the relevant emerging pathogens. Future research will hopefully help to find out if a shorter application time for the post contamination treatment of hands provides more benefits or more risks.
一些研究表明，手部污染后处理通常建议的30秒应用时间可能并非必要，因为某些配方在较短的应用时间（如15秒）内可能达到相同效果。
我们在时间杀灭试验中评估了一种乙醇基洗手液（施洁舒适凝胶）在15秒内对11种革兰氏阳性菌、16种革兰氏阴性菌和11种新出现的细菌病原体的杀菌活性。每种菌株均进行四次重复评估。
发现该洗手液（85%乙醇，w/w）在15秒内可使所有11种革兰氏阳性菌和所有16种革兰氏阴性菌减少超过5个对数10级，不仅针对美国典型培养物保藏中心（ATCC）测试菌株，也针对相应的临床分离株。此外，对所有测试的新出现细菌病原体均观察到对数10减少>5。
发现该乙醇基洗手液仅在15秒内具有广谱杀菌活性，包括引起医院感染的最常见菌种和相关新出现的病原体。未来的研究有望有助于查明手部污染后处理较短的应用时间是否会带来更多益处或更多风险。
