Ettler P, Martínková L, Ujcová E, Seichert L
Department of Biotechnology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Folia Microbiol (Praha). 1991;36(5):493-7. doi: 10.1007/BF02884071.
Problems associated with the development of submersed citric acid biosynthesis on sugar beet molasses for process improvement were solved by scale-down experiments: the determination of optimal temperature profile, optimal pH value, influence of OTR and inoculum quality. The optimal pH value at the start of the process was 6.4 with 3% of inoculum volume and temperature profile between 28-30 degrees C. In the case of OTR which is scale-dependent, we prefer to use DOC pilot plant data for the transfer of technology.
确定最佳温度曲线、最佳pH值、OTR的影响和接种物质量。该工艺开始时的最佳pH值为6.4，接种物体积为3%，温度曲线在28 - 30摄氏度之间。对于与规模相关的OTR，我们更倾向于使用中试工厂数据来进行技术转移。
