Romanenko Lyudmila A, Uchino Masataka, Tanaka Naoto, Frolova Galina M, Mikhailov Valery V
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):370-4. doi: 10.1099/ijs.0.65391-0.
An aerobic, Gram-negative bacterium, strain KMM 329(T), was isolated from a deep-sea sponge specimen from the Philippine Sea and subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis showed that strain KMM 329(T) clustered with the species of the genus Lysobacter. The highest level of 16S rRNA gene sequence similarity (97.0 %) was found with respect to Lysobacter concretionis KCTC 12205(T); lower values (96.4-95.2 %) were obtained with respect to the other recognized Lysobacter species. The value for DNA-DNA relatedness between strain KMM 329(T) and L. concretionis KCTC 12205(T) was 47 %. Branched fatty acids 16 : 0 iso, 15 : 0 iso, 11 : 0 iso 3-OH and 17 : 1 iso were found to be predominant. Strain KMM 329(T) had a DNA G+C content of 69.0 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain KMM 329(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter spongiicola sp. nov. is proposed. The type strain is KMM 329(T) (=NRIC 0728(T) =JCM 14760(T)).
从菲律宾海的深海海绵标本中分离出一株需氧革兰氏阴性菌，菌株KMM 329(T)，并对其进行了多相分类学研究。比较16S rRNA基因序列分析表明，菌株KMM 329(T)与溶杆菌属的物种聚类在一起。发现与凝结溶杆菌KCTC 12205(T)的16S rRNA基因序列相似性最高(97.0%)；与其他已确认的溶杆菌属物种的相似性较低(96.4 - 95.2%)。菌株KMM 329(T)与凝结溶杆菌KCTC 12205(T)之间的DNA - DNA相关性值为47%。发现支链脂肪酸16 : 0异、15 : 0异、11 : 0异3 - OH和17 : 1异占主导地位。菌株KMM 329(T)的DNA G + C含量为69.0 mol%。基于表型、化学分类学、DNA - DNA杂交和系统发育数据，菌株KMM 329(T)代表溶杆菌属的一个新物种，为此提出名称溶杆菌海绵icola sp. nov.。模式菌株为KMM 329(T)(=NRIC 0728(T)=JCM 14760(T))。
