Cui J F, Niu C Q, Zhang J S
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1991;26(11):852-7.
Six Ephedra alkaloids, namely ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine and methylpseudoephedrine, in 12 species of Chinese Ephedra were successfully separated and determined by gas chromatography with the highly specific and sensitive nitrogen phosphorus detector (GC/NPD). The column used (HP-5) had a cross linked 5% phenylmethylsilicone phase. Diphenylamine was used as the internal standard to check the reproducibility of the extraction yields of the alkaloids, the stability of the detector response and to quantify the alkaloids. The contents of the six alkaloids were calculated according to their regression equations. The way for the preparation of crude drug samples was improved, the diethyl ether extract of the alkalized crude sample was directly analysed by GC. The method is simple, rapid and sensitive. The results are in agreement with those of the HPLC method.
采用具有高特异性和高灵敏度的氮磷检测器（GC/NPD）的气相色谱法，成功分离并测定了12种中国麻黄属植物中的6种麻黄生物碱，即麻黄碱、伪麻黄碱、去甲麻黄碱、去甲伪麻黄碱、甲基麻黄碱和甲基伪麻黄碱。所用色谱柱（HP-5）为交联5%苯基甲基硅氧烷固定相。以二苯胺为内标，考察生物碱提取率的重现性、检测器响应的稳定性并对生物碱进行定量。根据回归方程计算6种生物碱的含量。改进了药材样品的制备方法，碱化后的药材粗品的乙醚提取物直接进行GC分析。该方法简便、快速、灵敏。结果与HPLC法一致。
