Yeh E, Driscoll R, Coltrera M, Olins A, Bloom K
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Nature. 1991 Feb 21;349(6311):713-5. doi: 10.1038/349713a0.
The tightly centromere-linked gene SPO15 is essential for meiotic cell division in the yeast Saccharomyces cerevisiae. Diploid cells without the intact SPO15 gene product are able to complete premeiotic DNA synthesis and genetic recombination, but are unable to traverse the division cycles. Electron microscopy of blocked cells reveals a duplicated but unseparated spindle-pole body. Thus cells are unable to form a bipolar spindle. Sequence analysis of SPO15 DNA reveals an open reading frame that predicts a protein of 704 amino acids. This protein is identical to VPS1, a gene involved in vacuolar protein sorting in yeast which has significant sequence homology (45% overall, 66% over 300 amino acids) to the microtubule bundling-protein, dynamin. The SPO15 gene product expressed in Escherichia coli can be affinity-purified with microtubules. SPO15 encodes a protein that is likely to be involved in a microtubule-dependent process required for the timely separation of spindle-pole bodies in meiosis.
紧密连接着丝粒的基因SPO15对于酿酒酵母的减数分裂细胞分裂至关重要。没有完整SPO15基因产物的二倍体细胞能够完成减数分裂前的DNA合成和基因重组，但无法经历分裂周期。对受阻细胞的电子显微镜观察显示有一个复制但未分离的纺锤极体。因此细胞无法形成双极纺锤体。SPO15 DNA的序列分析揭示了一个开放阅读框，预测其编码一个704个氨基酸的蛋白质。该蛋白质与VPS1相同，VPS1是酵母中参与液泡蛋白分选的一个基因，它与微管成束蛋白发动蛋白具有显著的序列同源性（总体为45%，在300个氨基酸上为66%）。在大肠杆菌中表达的SPO15基因产物可以用微管进行亲和纯化。SPO15编码一种可能参与减数分裂中纺锤极体及时分离所需的微管依赖性过程的蛋白质。
