Zhang Hui, Zhang Da-liang, Li Rui-feng, Guo Cheng-hao
Department of Otorhinolaryngology, Second Hospital of Shandong University, Jinan 250033, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2003 Feb;38(1):32-4.
To investigate the relationship between expression of leukocyte inducible nitric oxide synthase (iNOS)-mRNA and allergic rhinitis (AR).
Thirty-five patients with AR and 30 healthy controls were included in this study. Expression of iNOS-mRNA in peripheral blood leukocyte was detected by in situ hybridization. NO in plasm was measured by nitrate reductase. Expression of iNOS-mRNA in nasal mucosal was detected in 8 patients with AR and 6 healthy controls.
No expression of leukocyte iNOS-mRNA in healthy controls was found. In AR patients, the positive cells were significantly increased, the positive rate reached 40.82%. Expression of iNOS-mRNA was localized at the epithelium, gland and macrophage in healthy controls. Hyperplasia and expression of iNOS-mRNA increased at epithelium, gland and macrophage in the AR patients(t = 23.17, P < 0.001). The level of plasm NO in AR group was higher than that in healthy control group (t = 27.89, P < 0.001).
There is a relationship between expression of leukocyte iNOS-mRNA and the level of plasm NO in AR patients. The study provides an easy method of in situ hybridization for detecting some signal in body.
探讨白细胞诱导型一氧化氮合酶（iNOS）-mRNA表达与变应性鼻炎（AR）的关系。
本研究纳入35例AR患者和30例健康对照。采用原位杂交法检测外周血白细胞中iNOS-mRNA的表达。用硝酸还原酶法测定血浆中NO。选取8例AR患者和6例健康对照检测鼻黏膜中iNOS-mRNA的表达。
健康对照中未发现白细胞iNOS-mRNA表达。AR患者中，阳性细胞显著增多，阳性率达40.82%。健康对照中iNOS-mRNA表达定位于上皮、腺体和巨噬细胞。AR患者上皮、腺体和巨噬细胞中iNOS-mRNA的增生及表达增加（t = 23.17，P < 0.001）。AR组血浆NO水平高于健康对照组（t = 27.89，P < 0.001）。
AR患者白细胞iNOS-mRNA表达与血浆NO水平之间存在关联。本研究提供了一种简便的原位杂交方法用于检测体内某些信号。
