Yang H M, Chaowagul W, Sokol P A
Department of Microbiology and Infectious Diseases, University of Calgary Health Science Centre, Alberta, Canada.
Infect Immun. 1991 Mar;59(3):776-80. doi: 10.1128/iai.59.3.776-780.1991.
Eighty-four strains of Pseudomonas pseudomallei isolated from patients with melioidosis were examined for siderophore production. All the strains were shown to produce siderophore both on chrome azurol S agar plates and in liquid medium under iron-deficient conditions. Chemical assays indicated that the siderophore belongs to the hydroxamate class. Addition of iron to the culture medium resulted in increased culture growth with markedly decreased yield of siderophore. Siderophore produced by strain U7 was purified by gel filtration chromatography, and the molecular weight was estimated to be 1,000. When this partially purified siderophore was added to culture medium, it promoted iron uptake by P. pseudomallei in the presence of EDTA and enhanced growth of the organism in the presence of transferrin. We have given this siderophore the trivial name malleobactin.
对从类鼻疽患者中分离出的84株类鼻疽假单胞菌进行了铁载体产生情况检测。结果显示，所有菌株在缺铁条件下，于铬天青S琼脂平板和液体培养基中均能产生铁载体。化学分析表明，该铁载体属于异羟肟酸类。向培养基中添加铁会使培养物生长加快，同时铁载体产量显著降低。菌株U7产生的铁载体通过凝胶过滤色谱法进行纯化，其分子量估计为1000。当将这种部分纯化的铁载体添加到培养基中时，在乙二胺四乙酸（EDTA）存在的情况下，它能促进类鼻疽假单胞菌对铁的摄取，并在转铁蛋白存在的情况下增强该菌的生长。我们赋予这种铁载体俗名“类鼻疽菌素”。
