用来自同源和异源的脱氧核糖核酸对淋病奈瑟菌的亮氨酸和利福平性状进行转化
Transformation of leucine and rifampin traits in Neisseria gonorrhoeae with deoxyribonucleic acid from homologous and heterologous origins.
作者信息
Wood D O, Brownell G H
出版信息
J Bacteriol. 1975 Feb;121(2):471-4. doi: 10.1128/jb.121.2.471-474.1975.
Abstract
A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less than that of the homologous gonococcal DNA. A chemically defined medium that would support growth of most gonococcal isolates was used as a complete medium. A minimal medium was used for selection of Leu+ transformants. N-methyl-N'-nitro-N-nitrosoguanidine was used as a mutagen for isolating leucine prototrophs from leucine-requiring isolates of N. gonorrohoeae.
摘要
一株需要亮氨酸、对利福平敏感的淋病奈瑟菌菌株，用从脑膜炎奈瑟菌和淋病奈瑟菌获得的脱氧核糖核酸（DNA）转化为不需要亮氨酸、对利福平耐药的表型。脑膜炎球菌DNA的转化效率比对同源淋病球菌DNA的转化效率低约10至100倍。一种能支持大多数淋病球菌分离株生长的化学成分确定的培养基用作完全培养基。用基本培养基来选择亮氨酸营养缺陷型转化体。N-甲基-N'-硝基-N-亚硝基胍用作诱变剂，从需要亮氨酸的淋病奈瑟菌分离株中分离亮氨酸原养型。
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