Snider D E, Parker C W
J Clin Invest. 1976 Aug;58(2):524-7. doi: 10.1172/JCI108496.
In purified lymphocytes from the peripheral blood of healthy human subjects who had ingested therapeutic doses of aspirin, there was a significant decrease in resting cyclic AMP levels as well as a partial inhibition of the rise in cyclic AMP with isoproterenol or prostaglandin E1. These changes were seen as early as 30 min after aspirin ingestion and did not appear to result from aspirin effects on lymphocyte recovery, purity, viability, or relative number of thymus- or bone marrow-derived lymphocytes. In contrast, the direct addition of aspirin to suspensions of purified peripheral lymphocytes did not significantly alter their cyclic AMP levels. However, an effect of aspirin could be obtained in vitro if aspirin was added to unprocessed whole blood during the dextran sedimentation phase of the cell purification. Thus the effect of aspirin on lymphocyte cyclic AMP metabolism, may be indirect, through other cells present in the peripheral blood.
在摄入治疗剂量阿司匹林的健康人体受试者外周血纯化淋巴细胞中，静息环磷酸腺苷（cAMP）水平显著降低，同时异丙肾上腺素或前列腺素E1引起的cAMP升高受到部分抑制。这些变化在摄入阿司匹林后30分钟就已出现，似乎并非由阿司匹林对淋巴细胞恢复、纯度、活力或胸腺或骨髓来源淋巴细胞相对数量的影响所致。相比之下，将阿司匹林直接添加到纯化外周淋巴细胞悬液中，并未显著改变其cAMP水平。然而，如果在细胞纯化的右旋糖酐沉降阶段将阿司匹林添加到未处理的全血中，在体外可获得阿司匹林的效应。因此，阿司匹林对淋巴细胞cAMP代谢的影响可能是间接的，通过外周血中存在的其他细胞介导。
