Wang Jun, Ma Ting, Zhao Lingxia, Lv Jinghua, Li Guoqiang, Zhang Hao, Zhao Ben, Liang Fenglai, Liu Rulin
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, People's Republic of China.
J Ind Microbiol Biotechnol. 2008 Jun;35(6):619-28. doi: 10.1007/s10295-008-0326-9. Epub 2008 Feb 14.
A field experiment was performed to monitor changes in exogenous bacteria and to investigate the diversity of indigenous bacteria during a field trial of microbial enhanced oil recovery (MEOR). Two wells (26-195 and 27-221) were injected with three exogenous strains and then closed to allow for microbial growth and metabolism. After a waiting period, the pumps were restarted and the samples were collected. The bacterial populations of these samples were analyzed by denaturing gradient gel electrophoresis (DGGE) with PCR-amplified 16S rRNA fragments. DGGE profiles indicated that the exogenous strains were retrieved in the production water samples and indigenous strains could also be detected. After the pumps were restarted, average oil yield increased to 1.58 and 4.52 tons per day in wells 26-195 and 27-221, respectively, compared with almost no oil output before the injection of exogenous bacteria. Exogenous bacteria and indigenous bacteria contributed together to the increased oil output. Sequence analysis of the DGGE bands revealed that Proteobacteria were a major component of the predominant bacteria in both wells. Changes in the bacteria population in the reservoirs during MEOR process were monitored by molecular analysis of the 16S rRNA gene sequence. DGGE analysis was a successful approach to investigate the changes in microorganisms used for enhancing oil recovery. The feasibility of MEOR technology in the petroleum industry was also demonstrated.
在微生物强化采油（MEOR）的现场试验中，进行了一项现场实验，以监测外源细菌的变化并研究本地细菌的多样性。向两口井（26 - 195和27 - 221）注入了三种外源菌株，然后关闭井口以促进微生物生长和代谢。经过一段等待期后，重新启动泵并采集样品。通过对PCR扩增的16S rRNA片段进行变性梯度凝胶电泳（DGGE）分析这些样品的细菌种群。DGGE图谱表明，在采出水样品中检测到了外源菌株，同时也能检测到本地菌株。重新启动泵后，与注入外源细菌前几乎没有产油相比，26 - 195井和27 - 221井的平均日产油量分别增加到了1.58吨和4.52吨。外源细菌和本地细菌共同促成了产油量的增加。DGGE条带的序列分析表明，变形菌门是两口井中优势细菌的主要组成部分。通过对16S rRNA基因序列的分子分析监测了MEOR过程中油藏内细菌种群的变化。DGGE分析是研究用于提高采油率的微生物变化的一种成功方法。同时也证明了MEOR技术在石油工业中的可行性。
