Takeda Y, Miyamori I, Yoneda T, Takeda R
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Life Sci. 1991;48(26):2553-6. doi: 10.1016/0024-3205(91)90611-e.
Release of endothelin-1 (ET-1) from the mesenteric arteries of Wistar rats with streptozotocin-induced diabetes (STZ-DM) rats and nondiabetic rats was measured by a specific enzyme immunoassay following purification using an immunoaffinity column. The mesenteric arteries from STZ-DM rats released a significantly higher amount of ET-1 as compared to control rats (35.8 +/- 2.8 vs 14.9 +/- 2.0 pg/1hr, p less than 0.05). The plasma level of ET-1 in STZ-DM rats was also elevated to a significant extent as compared to controls (5.1 +/- 0.4 vs 3.0 +/- 0.4 pg/ml, p less than 0.05). The systolic blood pressure of STZ-DM rats was significantly higher than of the controls (p less than 0.05). The increased level of plasma ET-1 as well as its release from the mesenteric artery of STZ-DM rats may suggest its release following damage to the endothelium caused by diabetes and/or by associated changes in blood pressure.
使用免疫亲和柱纯化后，通过特定的酶免疫测定法测量链脲佐菌素诱导的糖尿病（STZ - DM）大鼠和非糖尿病大鼠的肠系膜动脉中内皮素 - 1（ET - 1）的释放量。与对照大鼠相比，STZ - DM大鼠的肠系膜动脉释放的ET - 1量显著更高（35.8±2.8对14.9±2.0 pg/1小时，p < 0.05）。与对照组相比，STZ - DM大鼠的血浆ET - 1水平也显著升高（5.1±0.4对3.0±0.4 pg/ml，p < 0.05）。STZ - DM大鼠的收缩压显著高于对照组（p < 0.05）。STZ - DM大鼠血浆ET - 1水平的升高及其从肠系膜动脉的释放可能表明，它是在糖尿病引起的内皮损伤和/或血压相关变化后释放的。
