Adam P, Sobeka O, Scott C S
Laboratory of Reference for Cerebrospinal Fluid and Neuroimmunology, Homolka Hospital, 150 30 Prague, Czechia.
Folia Microbiol (Praha). 2007;52(5):529-34. doi: 10.1007/BF02932115.
Sixty-five samples of cerebrospinal fluid (CSF) were evaluated using an automated cytoflow method with the CD-Sapphire hematology analyzer in order to investigate possible relationships between cell population patterns and diagnostic groups and better understand the biology of neurological disease. A basic panel of CD markers, including CD3/4/8/19/138/HLA-DR, was used to analyze CSF samples from clinical and laboratory confirmed cases of multiple sclerosis, neuroborreliosis, viral and bacterial neuroinfective diseases, malignant infiltrations of meninges and scavenger macrophagic reactions of the central nervous system. The principles of immune response and the contribution of cytological 'disease-related patterns' for these nosological entities are described. The distinct patterns of lymphocyte subpopulations in neuroborreliosis appear to be characteristic and could possibly serve as diagnostic indicators. Further verification and research will be necessary to clarify the significance and nature of CD4+ CD8+ positive subset in cerebrospinal fluid.
为了研究细胞群体模式与诊断组之间的可能关系，并更好地理解神经疾病的生物学特性，我们使用CD - 蓝宝石血液分析仪的自动细胞流式方法对65份脑脊液样本进行了评估。使用包括CD3/4/8/19/138/HLA - DR在内的一组基本CD标志物，分析了来自临床和实验室确诊的多发性硬化症、神经莱姆病、病毒和细菌性神经感染性疾病、脑膜恶性浸润以及中枢神经系统清除性巨噬细胞反应病例的脑脊液样本。描述了这些疾病实体的免疫反应原理和细胞学“疾病相关模式”的作用。神经莱姆病中淋巴细胞亚群的独特模式似乎具有特征性，可能作为诊断指标。需要进一步的验证和研究来阐明脑脊液中CD4 + CD8 + 阳性亚群的意义和性质。
