Koltai Hinanit, Weingarten-Baror Carmiya
Department of Ornamental Horticulture, ARO Volcani Center, Bet Dagan, Israel.
Nucleic Acids Res. 2008 Apr;36(7):2395-405. doi: 10.1093/nar/gkn087. Epub 2008 Feb 24.
Microarray-hybridization specificity is one of the main effectors of microarray result quality. In the present review, we suggest a definition for specificity that spans four hybridization levels, from the single probe to the microarray platform. For increased hybridization specificity, it is important to quantify the extent of the specificity at each of these levels, and correct the data accordingly. We outline possible effects of low hybridization specificity on the obtained results and list possible effectors of hybridization specificity. In addition, we discuss several studies in which theoretical approaches, empirical means or data filtration were used to identify specificity effectors, and increase the specificity of the hybridization results. However, these various approaches may not yet provide an ultimate solution; rather, further tool development is needed to enhance microarray-hybridization specificity.
微阵列杂交特异性是影响微阵列结果质量的主要因素之一。在本综述中，我们提出了一个涵盖从单个探针到微阵列平台四个杂交水平的特异性定义。为了提高杂交特异性，量化每个水平的特异性程度并相应地校正数据非常重要。我们概述了低杂交特异性对所得结果可能产生的影响，并列出了杂交特异性的可能影响因素。此外，我们讨论了几项使用理论方法、实证手段或数据过滤来识别特异性影响因素并提高杂交结果特异性的研究。然而，这些不同的方法可能尚未提供最终解决方案；相反，需要进一步开发工具来提高微阵列杂交特异性。
