Debartolomeis J, Cabelli V J
Department of Microbiology, University of Rhode Island, Kingston 02881-0812.
Appl Environ Microbiol. 1991 May;57(5):1301-5. doi: 10.1128/aem.57.5.1301-1305.1991.
A method was developed for the selective enumeration of F male-specific bacteriophages in samples of environmental waters. The host strain for the phages, Escherichia coli HS(pFamp)R, has three antibiotic resistance markers, ampicillin on the Famp plasmid, which codes for pilus production, and streptomycin and nalidixic acid on the chromosome. The strain is resistant to coliphages T2 to T7 and phi X174. More than 95% of the phages from environmental samples which plaqued on the host strain were F male specific. The host bacterium had a higher plaquing efficiency than E. coli K-12 Hfr for F-specific phages in stock suspensions and sewage effluents. The F male-specific phage levels in prechlorinated, secondary-treated sewage effluents generally were about 10(3) to 10(4) PFU/100 ml. The levels in the influents to the sewage treatment plants and in septic tank contents were about 10(5) PFU/100 ml. RNA-containing phages composed about 90% of the total F-specific phage population in sewage effluents.
开发了一种用于选择性计数环境水样中F雄性特异性噬菌体的方法。噬菌体的宿主菌株大肠杆菌HS(pFamp)R具有三种抗生素抗性标记，Famp质粒上的氨苄青霉素（其编码菌毛产生）以及染色体上的链霉素和萘啶酸。该菌株对大肠杆菌噬菌体T2至T7和phi X174具有抗性。在宿主菌株上形成噬菌斑的环境样品中，超过95%的噬菌体是F雄性特异性的。对于储备悬浮液和污水中的F特异性噬菌体，宿主细菌比大肠杆菌K-12 Hfr具有更高的噬菌斑形成效率。预氯化的二级处理污水中F雄性特异性噬菌体水平通常约为10³至10⁴ PFU/100 ml。污水处理厂进水和化粪池内容物中的水平约为10⁵ PFU/100 ml。含RNA的噬菌体约占污水中总F特异性噬菌体群体的90%。
