Osawa S, Furuse K, Watanabe I
Appl Environ Microbiol. 1981 Jan;41(1):164-8. doi: 10.1128/aem.41.1.164-168.1981.
To determine the distribution pattern of ribonucleic acid (RNA) coliphages (classified by serological groups I through IV) in animal sources, we isolated RNA phages from (i) feces samples from domestic animals (cows, pigs, horses, and fowls), some other animals in a zoological garden, and humans, (ii) the gastrointestinal contents of cows and pigs, and (iii) sewage samples from treatment plants in slaughter houses. These samples were then analyzed serologically. The concentration of RNA phages in the first and second kinds of material was fairly low (10 to 10(3) plaque-forming units per original phage sample), whereas that in the third kind of material was fairly high (10(3) to 10(5) plaque-forming units per original phage sample). Concerning the group types of the RNA phages in the first and second kinds of material, human feces contained RNA phages of groups II and III almost equally, the gastrointestinal contents of pigs included those of groups I and II equally, and the feces or gastrointestinal contents of other mammals other than humans and pigs had those of group I exclusively. In the third type of material we found mostly group I phages with a minor fraction of group II phages. Thus, the prominent features of the distribution pattern of RNA phages are the predominance of groups III and II in humans and the predominance of group I in animals.
为确定动物源中核糖核酸（RNA）噬菌体（按血清学分类为I至IV组）的分布模式，我们从以下样本中分离RNA噬菌体：（i）家畜（牛、猪、马和家禽）、动物园中的一些其他动物以及人类的粪便样本；（ii）牛和猪的胃肠道内容物；（iii）屠宰场处理厂的污水样本。然后对这些样本进行血清学分析。第一类和第二类样本中RNA噬菌体的浓度相当低（每个原始噬菌体样本有10至10³个噬菌斑形成单位），而第三类样本中的浓度相当高（每个原始噬菌体样本有10³至10⁵个噬菌斑形成单位）。关于第一类和第二类样本中RNA噬菌体的组型，人类粪便中II组和III组的RNA噬菌体含量几乎相等，猪的胃肠道内容物中I组和II组的噬菌体含量相等，除人类和猪以外的其他哺乳动物的粪便或胃肠道内容物中仅含有I组噬菌体。在第三类样本中，我们发现主要是I组噬菌体，还有一小部分II组噬菌体。因此，RNA噬菌体分布模式的突出特点是人类中III组和II组占优势，动物中I组占优势。
