Song Jeong-Sub, Jeong Hyun-Ja, Kim Su-Jin, Son Mu-Song, Na Ho-Jeong, Song Yong-Sun, Hong Seung-Heon, Kim Hyung-Min, Um Jae-Young
Professional Graduate School of Oriental Medicine, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Am J Chin Med. 2008;36(1):71-80. doi: 10.1142/S0192415X0800559X.
Interleukin-1 (IL-1), a cytokine produced predominantly by cells from the macrophage lineage, can affect multiple neuroendocrine and metabolic functions. IL-1alpha production by cultured peripheral blood mononuclear cells from an obese group was significantly elevated in comparison to a control group. The aim of this study was to investigate whether the IL-1alpha polymorphism and Sasang constitution, a major branch in Korean traditional medicine, were related to obesity. Genotyping was done in 182 healthy females with a marked variation in body mass index (BMI) by a PCR-restriction fragment length polymorphism assay. The T allele was associated with decreased BMI (p = 0.020). In a subgroup with BMI values ranging from 27 approximately 29 kg/m(2), the frequency of the T allele was significantly decreased (p = 0.004, odds ratio, OR = 0.141 compared to a subgroup with a BMI values less than 25 Kg/m(2)). In addition, in Taeumin female subjects, the frequency of the IL-1alpha T allele was markedly decreased in a subgroup with BMI values in the range of 27 approximately 29 kg/m(2) compared to a lean group with BMI values less than 25 kg/m(2) (p = 0.004, OR = 0.139). In Korean women, an association was found between -889C/T polymorphism in the regulatory region of the IL-1alpha gene and BMI values. In addition, an association was found among IL-1alpha polymorphism, obesity, and the Sasang constitution.
白细胞介素-1（IL-1）是一种主要由巨噬细胞系细胞产生的细胞因子，可影响多种神经内分泌和代谢功能。与对照组相比，肥胖组培养的外周血单核细胞产生的IL-1α显著升高。本研究的目的是调查IL-1α基因多态性与韩国传统医学主要分支之一的体质类型是否与肥胖有关。通过聚合酶链反应-限制性片段长度多态性分析对182名体重指数（BMI）有显著差异的健康女性进行基因分型。T等位基因与BMI降低有关（p = 0.020）。在BMI值范围为27至29 kg/m²的亚组中，T等位基因的频率显著降低（p = 0.004，优势比，与BMI值小于25 Kg/m²的亚组相比，OR = 0.141）。此外，在太阴体质女性受试者中，与BMI值小于25 kg/m²的瘦组相比，BMI值在27至29 kg/m²范围内的亚组中IL-1α T等位基因的频率显著降低（p = 0.004，OR = 0.139）。在韩国女性中，发现IL-1α基因调控区的-889C/T多态性与BMI值之间存在关联。此外，还发现IL-1α基因多态性、肥胖和体质类型之间存在关联。
