Lee Jung-Bok, Sohn Ho-Yong, Shin Kee-Sun, Kim Jong-Sik, Jo Min-Sub, Jeon Chun-Pyo, Jang Jong-Ok, Kim Jang-Eok, Kwon Gi-Seok
School of Bioresource Sciences, Andong National University, Andong 760-749, Korea.
J Microbiol Biotechnol. 2008 Feb;18(2):343-9.
Vinclozolin, an endocrine disrupting chemical, is a chlorinated fungicide widely used to control fungal diseases. However, its metabolite 3,5-dichloroaniline is more toxic and persistent than the parent vinclozolin. For the biodegradation of vinclozolin, vinclozolin- and/or 3,5-dichloroaniline-degrading bacteria were isolated from pesticide-polluted agriculture soil. Among the isolated bacteria, a Rhodococcus sp. was identified from a 16S rDNA sequence analysis and named Rhodococcus sp. T1-1. The degradation ratios for vinclozolin or 3,5- dichloroaniline in a minimal medium containing vinclozolin (200 microg/ml) or 3,5-dichloroaniline (120 microg/ml) were 90% and 84.1%, respectively. Moreover, Rhodococcus sp. T1-1 also showed an effective capability to biodegrade dichloroaniline isomers on enrichment cultures in which they were contained. Therefore, these results suggest that Rhodococcus sp. T1-1 can bioremediate vinclozolin as well as 3,5-dichloroaniline.
乙烯菌核利是一种内分泌干扰化学物质，是一种广泛用于控制真菌病害的氯化杀菌剂。然而，其代谢产物3,5-二氯苯胺比母体乙烯菌核利毒性更强且更持久。为了实现乙烯菌核利的生物降解，从受农药污染的农业土壤中分离出了能降解乙烯菌核利和/或3,5-二氯苯胺的细菌。在分离出的细菌中，通过16S rDNA序列分析鉴定出一株红球菌属细菌，并将其命名为红球菌属T1-1。在含有乙烯菌核利（200微克/毫升）或3,5-二氯苯胺（120微克/毫升）的基本培养基中，乙烯菌核利或3,5-二氯苯胺的降解率分别为90%和84.1%。此外，红球菌属T1-1在含有二氯苯胺异构体的富集培养物中也表现出有效降解二氯苯胺异构体的能力。因此，这些结果表明红球菌属T1-1可以对乙烯菌核利以及3,5-二氯苯胺进行生物修复。
