Benabdelmoumene S, Dumont S, Petit C, Poindron P, Wachsmann D, Klein J P
Faculté de Chirurgie Dentaire, Unité INSERM 157, Strasbourg, France.
Infect Immun. 1991 Sep;59(9):3261-6. doi: 10.1128/iai.59.9.3261-3266.1991.
Streptococcus mutans serotype f polysaccharide (poly f) was prepared from S. mutans whole cells by autoclaving. The poly f was purified by chromatography on DEAE Trisacryl M and Bio-Gel P100, treated with insoluble pronase, and resubjected to chromatography on DEAE Trisacryl M. Normal human blood monocytes, stimulated in vitro with purified poly f, produced extracellular tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in a dose-dependent fashion as determined by a heterologous two-site sandwich enzyme-linked immunosorbent assay. Poly f also increased the expression of monocyte cell surface receptors for the Fc part of human immunoglobulin G, activity which is correlated with an increase of the phagocytic activity of the stimulated monocytes. Polymyxin B had no effect on TNF-alpha and IL-1 beta release. Neutralization assays with anti-recombinant human TNF-alpha and anti-recombinant human IL-1 beta immunoglobulin G confirmed the fact that the cytotoxic and mitogenic mediators released by the poly f-stimulated monocytes were mainly TNF-alpha and IL-1 beta.
变形链球菌血清型f多糖（多聚f）通过对变形链球菌全细胞进行高压灭菌制备。多聚f经DEAE Trisacryl M和Bio-Gel P100柱层析纯化，用不溶性链霉蛋白酶处理，然后再次进行DEAE Trisacryl M柱层析。用纯化的多聚f体外刺激正常人血液单核细胞，通过异源双位点夹心酶联免疫吸附测定法确定，其以剂量依赖方式产生细胞外肿瘤坏死因子α（TNF-α）和白细胞介素-1β（IL-1β）。多聚f还增加了人免疫球蛋白G Fc部分的单核细胞表面受体的表达，该活性与受刺激单核细胞吞噬活性的增加相关。多粘菌素B对TNF-α和IL-1β的释放无影响。用抗重组人TNF-α和抗重组人IL-1β免疫球蛋白G进行的中和试验证实了以下事实：多聚f刺激的单核细胞释放的细胞毒性和促有丝分裂介质主要是TNF-α和IL-1β。
