雌激素受体在母鸡输卵管染色质亚组分中的分布

Distribution of estrogen receptors in subfractions of hen oviduct chromatin.

作者信息

Hemminki K

出版信息

Nucleic Acids Res. 1976 Jun;3(6):1499-506. doi: 10.1093/nar/3.6.1499.

DOI:10.1093/nar/3.6.1499

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343008/

Abstract

Hen oviduct chromatin was digested with DNase II and separated into two fractions. The MgCl2 insoluble chromatin fraction (43% of the total DNA) was enriched in nucleosome-like particles, which sedimented at 11 S and contained 185 base pairs of DNA. The MgCl2 soluble chromatin fraction (5% of the total DNA) was characterized by 5 S and 14 S peaks in sucrose gradients; Estrogen receptors in the chromatin fractions were labelled with [3H] estradiol using the steroid exchange assay. The concentration of receptors in the MgCl2 soluble chromatin was 4;5 times higher than that in the MgCl2 insoluble chromatinmin sucrose gradient analysis the 11 S particles displayed a negligible specific radioactivity suggesting that estrogen receptors mainly bind to extranucleosomal chromatin.

摘要

用脱氧核糖核酸酶II消化母鸡输卵管染色质，并将其分离成两个组分。氯化镁不溶性染色质组分（占总DNA的43%）富含核小体样颗粒，这些颗粒在11S处沉降，含有185个碱基对的DNA。氯化镁可溶性染色质组分（占总DNA的5%）在蔗糖梯度中表现为5S和14S峰；使用类固醇交换测定法，用[3H]雌二醇标记染色质组分中的雌激素受体。在蔗糖梯度分析中，氯化镁可溶性染色质中受体的浓度比氯化镁不溶性染色质中的浓度高4.5倍，11S颗粒显示出可忽略不计的比放射性，这表明雌激素受体主要与核小体外染色质结合。

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本文引用的文献

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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸

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Nat New Biol. 1972 Dec 6;240(101):185-7. doi: 10.1038/newbio240185a0.

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Spheroid chromatin units (v bodies).球状染色质单位（V小体）。

Science. 1974 Jan 25;183(4122):330-2. doi: 10.1126/science.183.4122.330.

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