Graduate Program of Health Sciences, State University of Maringa, Parana, Brazil.
Evid Based Complement Alternat Med. 2011;2011:201953. doi: 10.1093/ecam/neq029. Epub 2011 Mar 9.
Propolis, a resinous compound produced by Apis mellifera L. bees, is known to possess a variety of biological activities and is applied in the therapy of various infectious diseases. The aim of this study was to evaluate the in vitro antifungal activity of propolis ethanol extract (PE) and propolis microparticles (PMs) obtained from a sample of Brazilian propolis against clinical yeast isolates of importance in the vulvovaginal candidiasis (VVC). PE was used to prepare the microparticles. Yeast isolates (n = 89), obtained from vaginal exudates of patients with VVC, were exposed to the PE and the PMs. Moreover, the main antifungal drugs used in the treatment of VVC (Fluconazole, Voriconazole, Itraconazole, Ketoconazole, Miconazole and Amphotericin B) were also tested. Minimum inhibitory concentration (MIC) was determined according to the standard broth microdilution method. Some Candida albicans isolates showed resistance or dose-dependent susceptibility for the azolic drugs and Amphotericin B. Non-C. albicans isolates showed more resistance and dose-dependent susceptibility for the azolic drugs than C. albicans. However, all of them were sensitive or dose-dependent susceptible for Amphotericin B. All yeasts were inhibited by PE and PMs, with small variation, independent of the species of yeast. The overall results provided important information for the potential application of PMs in the therapy of VVC and the possible prevention of the occurrence of new symptomatic episodes.
蜂胶，一种由蜜蜂 Apis mellifera L. 产生的树脂状化合物，已知具有多种生物活性，并应用于各种感染性疾病的治疗。本研究旨在评估来自巴西蜂胶样本的蜂胶乙醇提取物 (PE) 和蜂胶微粒 (PMs) 的体外抗真菌活性，以对抗阴道念珠菌病 (VVC) 中重要的临床酵母分离株。PE 用于制备微粒。从 VVC 患者的阴道分泌物中分离出的 89 个酵母分离株（n=89）暴露于 PE 和 PMs 中。此外，还测试了治疗 VVC 中使用的主要抗真菌药物（氟康唑、伏立康唑、伊曲康唑、酮康唑、咪康唑和两性霉素 B）。根据标准肉汤微量稀释法测定最小抑菌浓度 (MIC)。一些白色念珠菌分离株对唑类药物和两性霉素 B 表现出耐药性或剂量依赖性敏感性。非白色念珠菌分离株对唑类药物的耐药性和剂量依赖性敏感性高于白色念珠菌。然而，它们对两性霉素 B 均敏感或剂量依赖性敏感。所有酵母均被 PE 和 PMs 抑制，变化很小，与酵母的种类无关。总体结果为 PMs 在 VVC 治疗中的潜在应用以及可能预防新的症状发作提供了重要信息。
