Katz J, Chegini N, Shiverick K T, Lamont R J
Department of Oral Maxillofacial Surgery and Diagnostic Sciences, University of Florida College of Dentistry, PO Box 100414, Gainesville, FL 32610, USA.
J Dent Res. 2009 Jun;88(6):575-8. doi: 10.1177/0022034509338032.
Increasing evidence suggests an association between periodontal disease and adverse pregnancy outcomes. Although infection is considered as a risk factor for preterm delivery, the localization of oral bacteria or their antigens in chorioamnionitis placental tissue has never been demonstrated. This study was devised to test the hypothesis that periodontal pathogens may be present and affect human placenta in cases of chorioamnionitis. Using immunocytochemistry, we have identified the presence of Porphyromonas gingivalis antigens in placental tissues. The antigens were detected in the placental syncytiotrophoblasts, chorionic trophoblasts, decidual cells, and amniotic epithelial cells, as well as the vascular cells. There was a substantial increase in immunostaining intensity of the tissues sectioned from women with chorioamnionitis compared to those experiencing normalterm pregnancy, p < 0.019 (Mann-Whitney test). These results suggest that P. gingivalis may commonly colonize placental tissue, and that the presence of the organism may contribute to preterm delivery.
越来越多的证据表明牙周疾病与不良妊娠结局之间存在关联。尽管感染被认为是早产的一个风险因素，但口腔细菌或其抗原在绒毛膜羊膜炎胎盘组织中的定位从未得到证实。本研究旨在验证以下假设：在绒毛膜羊膜炎病例中，牙周病原体可能存在并影响人胎盘。我们使用免疫细胞化学方法，在胎盘组织中鉴定出牙龈卟啉单胞菌抗原的存在。这些抗原在胎盘合体滋养层细胞、绒毛膜滋养层细胞、蜕膜细胞、羊膜上皮细胞以及血管细胞中均被检测到。与足月妊娠妇女的组织切片相比，绒毛膜羊膜炎妇女的组织切片免疫染色强度显著增加，p < 0.019（曼-惠特尼检验）。这些结果表明牙龈卟啉单胞菌可能经常定植于胎盘组织，并且该微生物的存在可能导致早产。
