Arstila P
Arch Virol. 1976;51(1-2):51-8. doi: 10.1007/BF01317833.
Radioactively-labelled VSV, both Indiana and New Jersey serotypes, were rapidly and efficiently adsorbed onto goose erythrocytes, but only within a narrow range of pH and at low temperatures. Under optimal conditions for adsorption, both infectivity and haemagglutinating activity of VSV were reduced. A basic difference between the two serotypes was identified: adsorption and haemagglutination of the Indiana serotype were stable even at pH 9.0 and at 37 degrees C, whereas New Jersey serotype was eluted slowly at +0 degrees C and at pH 6.4 and rapidly at a higher temperature and pH. The rapid elution offers a useful method for purification of New Jersey serotype. Particle counting of VSV showed that 10 to 50 virus particles were required for one PFU. Haemagglutination was not a sensitive method for assaying small amounts of VSV; 3--20X10(7) virus particles or more than 10(7) PFU corresponded to one HAU.
放射性标记的水疱性口炎病毒（VSV），包括印第安纳血清型和新泽西血清型，能快速且高效地吸附到鹅红细胞上，但这仅在狭窄的pH范围内且在低温下才会发生。在吸附的最佳条件下，VSV的感染性和血凝活性均会降低。已确定两种血清型之间的一个基本差异：即使在pH 9.0和37摄氏度时，印第安纳血清型的吸附和血凝作用依然稳定，而新泽西血清型在0摄氏度和pH 6.4时洗脱缓慢，在较高温度和pH下则快速洗脱。这种快速洗脱为新泽西血清型的纯化提供了一种有用的方法。VSV的颗粒计数显示，一个空斑形成单位（PFU）需要10到50个病毒颗粒。血凝不是检测少量VSV的灵敏方法；3 - 20×10⁷个病毒颗粒或超过10⁷个PFU相当于一个血凝单位（HAU）。
