新型隐球菌中3-羟基氧化脂质的分布及对乙酰水杨酸的敏感性

Distribution of 3-hydroxy oxylipins and acetylsalicylic acid sensitivity in Cryptococcus species.

作者信息

Sebolai Olihile M, Pohl Carolina H, Botes Piet J, van Wyk Pieter W J, Mzizi Refilwe, Swart Chantel W, Kock Johan L F

机构信息

Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Nelson Mandela Drive, P.O. Box 339, Bloemfontein, Free State 9301, South Africa.

出版信息

Can J Microbiol. 2008 Feb;54(2):111-8. doi: 10.1139/w07-116.

DOI:10.1139/w07-116

PMID:18388980

Abstract

Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed that growth of Cryptococcus species was inhibited by acetylsalicylic acid (which is known to inhibit mitochondrial function, including the production of 3-hydroxy oxylipins) at concentrations as low as 1 mmol/L. The results suggest that acetylsalicylic acid is effective in controlling the growth of tested pathogens, probably by targeting their mitochondria. This study further expands the known function of this anti-inflammatory drug as anti-fungal agent.

摘要

我们使用一种经过充分验证的、对3-羟基氧化脂质具有特异性的抗体，绘制了这些氧化脂质在选定的隐球菌（丝孢酵母属）物种中的分布图。免疫荧光显微镜研究表明，这些化合物沉积在细胞壁表面、附属物和芽痕上。体外研究表明，低至1 mmol/L的乙酰水杨酸（已知可抑制线粒体功能，包括3-羟基氧化脂质的产生）就能抑制隐球菌属的生长。结果表明，乙酰水杨酸可能通过靶向病原体的线粒体来有效控制受试病原体的生长。这项研究进一步拓展了这种抗炎药物作为抗真菌剂的已知功能。