Kripke M L, Norbury K C, Gruys E, Hibbs J B
Infect Immun. 1977 Jul;17(1):121-9. doi: 10.1128/iai.17.1.121-129.1977.
It has been reported that trypan blue treatment decreases the nonspecific resistance of mice to transplanted tumors and inhibits the in vitro cytotoxic activity of activated macrophages. We wished to determine whether this effect of trypan blue could be due to a selective inhibition of certain macrophage functions or whether it reflected a broader form of immunosuppression. We therefore tested the effects of trypan blue on a variety of immunological responses. Treatment of mice with trypan blue delayed their rejection of skin allografts and transplants of a highly antigenic syngeneic ultraviolet light-induced tumor. Trypan blue treatment of either donor or recipient decreased the local graft-versus-host reaction. Filtration of lymph node cells from trypan blue-treated donors on a nylon wool column before use in the graft-versus-host assay abrogated the depressive effect of trypan blue. A transient reduction in the blastogenic response of spleen cells to concanavalin A and lipopolysaccharide mitogens was observed after a single injection of trypan blue, but the response of lymph node cells was unaffected. The depressed response of splenic lymphocytes was not entirely reversed by removal of adherent cells. The primary and secondary hemagglutinin responses to sheep erythrocytes were unaffected in trypan blue-treated mice, and the proportion and phagocytic activity of thioglycolate-induced peritoneal macrophages were also unaltered. We conclude that treatment of mice with trypan blue selectively inhibits certain macrophage functions but, at high doses, it can also inhibit some lymphocyte activities.
据报道，锥虫蓝处理会降低小鼠对移植肿瘤的非特异性抵抗力，并抑制活化巨噬细胞的体外细胞毒性活性。我们希望确定锥虫蓝的这种作用是否可能是由于对某些巨噬细胞功能的选择性抑制，或者它是否反映了一种更广泛的免疫抑制形式。因此，我们测试了锥虫蓝对多种免疫反应的影响。用锥虫蓝处理小鼠会延迟它们对皮肤同种异体移植物和高抗原性同基因紫外线诱导肿瘤移植的排斥反应。对供体或受体进行锥虫蓝处理会降低局部移植物抗宿主反应。在用于移植物抗宿主试验之前，将来自经锥虫蓝处理的供体的淋巴结细胞在尼龙毛柱上过滤，可消除锥虫蓝的抑制作用。单次注射锥虫蓝后，观察到脾细胞对刀豆球蛋白A和脂多糖有丝分裂原的增殖反应短暂降低，但淋巴结细胞的反应未受影响。去除贴壁细胞并不能完全逆转脾淋巴细胞的反应降低。锥虫蓝处理的小鼠对绵羊红细胞的初次和二次血凝素反应未受影响，硫代乙醇酸盐诱导的腹腔巨噬细胞的比例和吞噬活性也未改变。我们得出结论，用锥虫蓝处理小鼠会选择性地抑制某些巨噬细胞功能，但在高剂量时，它也会抑制一些淋巴细胞活性。
