从红雀珊瑚中分离和鉴定抗氧化剂。

Isolation and identification of antioxidants from Pedilanthus tithymaloides.

作者信息

Abreu Pedro M, Matthew Susan, González Tania, Vanickova Lucie, Costa David, Gomes Ana, Segundo Marcela A, Fernandes Eduarda

机构信息

Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, CQFB/REQUIMTE, 2829-516, Caparica, Portugal.

出版信息

J Nat Med. 2008 Jan;62(1):67-70. doi: 10.1007/s11418-007-0186-z. Epub 2007 Jul 31.

DOI:10.1007/s11418-007-0186-z

PMID:18404345

Abstract

A bioassay-guided methodology utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), the trolox equivalent antioxidant capacity (TEAC), and reducing power assays, as well as an assessment of scavenging properties against O (2) (.-) , H(2)O(2), HOCl, ROO., .NO, and ONOO(-) were used to find the main antioxidant principles of Pedilanthus tithymaloides (Euphorbiaceae), a shrub used in traditional Cuban medicine. The principles were identified as kaempferol 3-O-beta-D-glucopyranoside-6''-(3-hydroxy-3-methylglutarate), quercitrin, isoquercitrin, and scopoletin. The contents of total phenolics and flavonoids were found to be 76.0 +/- 4.8 mg of gallic acid equivalents/g extract and 9.8 +/- 0.4 mg of rutin equivalents/g extract, respectively.

摘要

采用一种生物测定指导方法，利用2,2-二苯基-1-苦基肼（DPPH）、特洛克斯等效抗氧化能力（TEAC）和还原能力测定法，以及对超氧阴离子（O(2)(.-)）、过氧化氢（H(2)O(2)）、次氯酸（HOCl）、过氧自由基（ROO.）、一氧化氮（.NO）和过氧亚硝酸根（ONOO(-)）清除特性的评估，来寻找古巴传统医学中使用的一种灌木——银边翠（大戟科）的主要抗氧化成分。这些成分被鉴定为山奈酚3-O-β-D-吡喃葡萄糖苷-6''-(3-羟基-3-甲基戊二酸)、槲皮苷、异槲皮苷和东莨菪素。总酚和黄酮类化合物的含量分别为76.0±4.8毫克没食子酸当量/克提取物和9.8±0.4毫克芦丁当量/克提取物。