Musoke-Zawedde Patricia, Shoichet Molly S
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, M5S 3E5, Canada.
Biomed Mater. 2006 Sep;1(3):162-9. doi: 10.1088/1748-6041/1/3/011. Epub 2006 Aug 9.
The objective of this study was to investigate the neurite guidance potential of concentration gradients of glycine-arginine-glycine-aspartic acid-serine (GRGDS) oligopeptides immobilized within three-dimensional patterned cylindrical volumes created in a biodegradable nerve guidance matrix. This was achieved using ultraviolet (UV) laser micropatterning of a hyaluronan (HA) hydrogel matrix modified with S-2-nitrobenzyl cysteine. Upon exposure to focused laser light, the 2-nitrobenzyl group was cleaved, exposing thiol groups which reacted with maleimide-terminated GRGDS exclusively within these laser-defined volumes. We show that the UV laser micropatterning technique can be used to create GRGDS peptide concentration gradients within the oligopeptide channels and that these channels guide neurite outgrowth from primary neural cells.
本研究的目的是研究固定在可生物降解神经导向基质中创建的三维图案化圆柱形体积内的甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸（GRGDS）寡肽浓度梯度的神经突导向潜力。这是通过对用S-2-硝基苄基半胱氨酸修饰的透明质酸（HA）水凝胶基质进行紫外（UV）激光微图案化来实现的。在聚焦激光照射下，2-硝基苄基基团被裂解，暴露出硫醇基团，这些硫醇基团仅在这些激光定义的体积内与马来酰亚胺末端的GRGDS反应。我们表明，UV激光微图案化技术可用于在寡肽通道内创建GRGDS肽浓度梯度，并且这些通道可引导原代神经细胞的神经突生长。
