Robb Ellen L, Winkelmolen Lieke, Visanji Naomi, Brotchie Jonathan, Stuart Jeffrey A
Department of Biological Sciences, Brock University, 500 Glenridge Road, St. Catharines, Ont., Canada.
Biochem Biophys Res Commun. 2008 Jul 18;372(1):254-9. doi: 10.1016/j.bbrc.2008.05.028. Epub 2008 May 16.
trans-Resveratrol (3,4',5-trihydroxystilbene; RES) is of interest for its reported protective effects in a variety of pathologies, including neurodegeneration. Many of these protective properties have been attributed to the ability of RES to reduce oxidative stress. In vitro studies have shown an increase in antioxidant enzyme activities following exposure to RES, including upregulation of mitochondrial superoxide dismutase, an enzyme that is capable of reducing both oxidative stress and cell death. We sought to determine if a similar increase in endogenous antioxidant enzymes is observed with RES treatment in vivo. Three separate modes of RES delivery were utilized; in a standard diet, a high fat diet and through a subcutaneous osmotic minipump. RES given in a high fat diet proved to be effective in elevating antioxidant capacity in brain resulting in an increase in both MnSOD protein level (140%) and activity (75%). The increase in MnSOD was not due to a substantial proliferation of mitochondria, as RES treatment induced a 10% increase in mitochondrial abundance (Citrate Synthase activity). The potential neuroprotective properties of MnSOD have been well established, and we demonstrate that a dietary delivery of RES is able to increase the expression and activity of this enzyme in vivo.
反式白藜芦醇（3,4',5-三羟基茋；RES）因其在包括神经退行性变在内的多种病理状态下具有报道的保护作用而备受关注。这些保护特性中的许多都归因于RES降低氧化应激的能力。体外研究表明，暴露于RES后抗氧化酶活性增加，包括线粒体超氧化物歧化酶的上调，该酶能够减少氧化应激和细胞死亡。我们试图确定在体内用RES治疗是否能观察到内源性抗氧化酶有类似的增加。采用了三种不同的RES给药方式；在标准饮食、高脂饮食中以及通过皮下渗透微型泵给药。结果证明，高脂饮食中给予的RES能有效提高大脑中的抗氧化能力，导致锰超氧化物歧化酶（MnSOD）蛋白水平增加140%，活性增加75%。MnSOD的增加并非由于线粒体大量增殖，因为RES治疗使线粒体丰度（柠檬酸合酶活性）增加了10%。MnSOD的潜在神经保护特性已得到充分证实，并且我们证明饮食中给予RES能够在体内增加该酶的表达和活性。
