Meijer A, Morré S A, van den Brule A J, Savelkoul P H, Ossewaarde J M
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, 3720 BA Bilthoven, University Hospital Vrije Universiteit, 1081 HV Amsterdam, The Netherlands.
J Bacteriol. 1999 Aug;181(15):4469-75. doi: 10.1128/JB.181.15.4469-4475.1999.
The genomic relatedness of 19 Chlamydia pneumoniae isolates (17 from respiratory origin and 2 from atherosclerotic origin), 21 Chlamydia trachomatis isolates (all serovars from the human biovar, an isolate from the mouse biovar, and a porcine isolate), 6 Chlamydia psittaci isolates (5 avian isolates and 1 feline isolate), and 1 Chlamydia pecorum isolate was studied by analyzing genomic amplified fragment length polymorphism (AFLP) fingerprints. The AFLP procedure was adapted from a previously developed method for characterization of clinical C. trachomatis isolates. The fingerprints of all C. pneumoniae isolates were nearly identical, clustering together at a Dice similarity of 92.6% (+/- 1.6% standard deviation). The fingerprints of the C. trachomatis isolates of human, mouse, and swine origin were clearly distinct from each other. The fingerprints of the isolates from the human biovar could be divided into at least 12 different types when the presence or absence of specific bands was taken into account. The C. psittaci fingerprints could be divided into a parakeet, a pigeon, and a feline type. The fingerprint of C. pecorum was clearly distinct from all others. Cluster analysis of selected isolates from all species revealed groups other than those based on sequence data from single genes (in particular, omp1 and rRNA genes) but was in agreement with available DNA-DNA hybridization data. In conclusion, cluster analysis of AFLP fingerprints of representatives of all species provided suggestions for a grouping of chlamydiae based on the analysis of the whole genome. Furthermore, genomic AFLP analysis showed that the genome of C. pneumoniae is highly conserved and that no differences exist between isolates of respiratory and atherosclerotic origins.
通过分析基因组扩增片段长度多态性（AFLP）指纹图谱，研究了19株肺炎衣原体分离株（17株来自呼吸道，2株来自动脉粥样硬化病变）、21株沙眼衣原体分离株（所有人源生物变种的血清型、1株鼠源生物变种分离株和1株猪源分离株）、6株鹦鹉热衣原体分离株（5株禽源分离株和1株猫源分离株）以及1株猪衣原体分离株的基因组相关性。AFLP方法是在先前开发的用于临床沙眼衣原体分离株鉴定方法的基础上改进而来。所有肺炎衣原体分离株的指纹图谱几乎相同，在Dice相似度为92.6%（±1.6%标准差）时聚为一类。人源、鼠源和猪源的沙眼衣原体分离株的指纹图谱彼此明显不同。考虑特定条带的有无时，人源生物变种分离株的指纹图谱可分为至少12种不同类型。鹦鹉热衣原体的指纹图谱可分为长尾小鹦鹉型、鸽型和猫型。猪衣原体的指纹图谱与其他所有图谱明显不同。对所有物种的选定分离株进行聚类分析，揭示了除基于单个基因（特别是omp1和rRNA基因）序列数据之外的其他分组，但与现有的DNA - DNA杂交数据一致。总之，对所有物种代表的AFLP指纹图谱进行聚类分析，为基于全基因组分析的衣原体分组提供了建议。此外，基因组AFLP分析表明，肺炎衣原体的基因组高度保守，呼吸道来源和动脉粥样硬化来源的分离株之间不存在差异。
