Dalgård Christine, Nielsen Flemming, Morrow Jason D, Enghusen-Poulsen Henrik, Jonung Torbjörn, Hørder Mogens, de Maat Moniek P M
Research Unit of Biochemistry, Pharmacology and Genetics, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark.
Br J Nutr. 2009 Jan;101(2):263-9. doi: 10.1017/S0007114508995660. Epub 2008 May 28.
Inflammation and endothelial activation are associated with an increased risk of CVD and epidemiological evidence suggests an association between levels of markers of inflammation or endothelial activation and the intake of fruit. Also, vitamin E, a fat-soluble antioxidant, has anti-inflammatory properties. We performed a randomised 2 x 2 factorial, crossover trial to determine the effect of orange and blackcurrant juice (500 ml/d) and vitamin E (15 mg RRR-alpha-tocopherol/d) supplementation on markers of inflammation and endothelial activation in forty-eight patients with peripheral arterial disease. Patients were randomly allocated to two dietary supplements from the four possible combinations of juice and vitamin E: juice+vitamin E; juice+placebo; reference beverage (sugar drink)+vitamin E; and reference beverage+placebo. The supplementations were given for 28 d, separated by a 4-week wash-out period. Analysis of main effects showed that juice decreased C-reactive protein (CRP) by 11% and fibrinogen by 3% while the reference drink increased CRP by 13% and fibrinogen by 2% (P<0.008 and P<0.002, respectively). No significant differences were measured for IL-6 and the endothelial activation markers von Willebrand factor, tissue-plasminogen activator and plasmin activator inhibitor-1. Vitamin E supplementation had no significant effects on the various markers. We observed no significant interaction between juice and vitamin E. In this study, orange and blackcurrant juice reduced markers of inflammation, but not markers of endothelial activation, in patients with peripheral arterial disease, relative to sugar drinks.
炎症和内皮细胞激活与心血管疾病（CVD）风险增加相关，流行病学证据表明炎症或内皮细胞激活标志物水平与水果摄入量之间存在关联。此外，维生素E是一种脂溶性抗氧化剂，具有抗炎特性。我们进行了一项随机2×2析因交叉试验，以确定橙汁和黑加仑汁（500毫升/天）以及补充维生素E（15毫克RRR-α-生育酚/天）对48例外周动脉疾病患者炎症和内皮细胞激活标志物的影响。患者从果汁和维生素E的四种可能组合中随机分配到两种膳食补充剂：果汁+维生素E；果汁+安慰剂；参考饮料（含糖饮料）+维生素E；以及参考饮料+安慰剂。补充剂服用28天，中间有4周的洗脱期。主效应分析表明，果汁使C反应蛋白（CRP）降低11%，纤维蛋白原降低3%，而参考饮料使CRP升高13%，纤维蛋白原升高2%（分别为P<0.008和P<0.002）。白细胞介素-6以及内皮细胞激活标志物血管性血友病因子、组织纤溶酶原激活物和纤溶酶激活物抑制剂-1未测得显著差异。补充维生素E对各种标志物无显著影响。我们未观察到果汁和维生素E之间有显著相互作用。在本研究中，相对于含糖饮料，橙汁和黑加仑汁降低了外周动脉疾病患者的炎症标志物，但未降低内皮细胞激活标志物。
