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Genogroup I picobirnaviruses in pigs: evidence for genetic diversity and relatedness to human strains.猪中的基因组I微小双股RNA病毒：遗传多样性及与人类毒株相关性的证据
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2
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MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.MEGA4：分子进化遗传学分析（MEGA）软件版本4.0。
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Molecular epidemiology of human picobirnaviruses among children of a slum community in Kolkata, India.印度加尔各答一个贫民窟社区儿童中人类微小双股RNA病毒的分子流行病学
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[Detection of picobirnaviruses by electrophoresis of RNA in polyacrylamide gel].通过聚丙烯酰胺凝胶中RNA电泳检测微小双股RNA病毒
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Sequence heterogeneity among human picobirnaviruses detected in a gastroenteritis outbreak.在一次肠胃炎暴发中检测到的人类微小双股RNA病毒之间的序列异质性。
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猪微小双股RNA病毒的分子特征及特异性逆转录-聚合酶链反应检测方法的建立

Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay.

作者信息

Carruyo Gabriela M, Mateu Guaniri, Martínez Laura C, Pujol Flor H, Nates Silvia V, Liprandi Ferdinando, Ludert Juan E

机构信息

Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Argentina.

出版信息

J Clin Microbiol. 2008 Jul;46(7):2402-5. doi: 10.1128/JCM.00655-08. Epub 2008 May 28.

DOI:10.1128/JCM.00655-08
PMID:18508933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2446882/
Abstract

The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.

摘要

报道了猪微小双股RNA病毒（PBV）毒株全长基因组2片段的分子特征，以及用于检测粪便中该病毒的特异性逆转录-聚合酶链反应（RT-PCR）检测方法的开发。系统发育分析表明，所研究的猪分离株与属于基因组I的人类PBV的亲缘关系比与迄今描述的其他猪PBV更密切（同一性>85%）。对在委内瑞拉和阿根廷采集的粪便样本进行RT-PCR和聚丙烯酰胺凝胶电泳分析表明，PBV在仔猪中高频率传播。