猪微小双股RNA病毒的分子特征及特异性逆转录-聚合酶链反应检测方法的建立
Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay.
作者信息
Carruyo Gabriela M, Mateu Guaniri, Martínez Laura C, Pujol Flor H, Nates Silvia V, Liprandi Ferdinando, Ludert Juan E
机构信息
Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Argentina.
出版信息
J Clin Microbiol. 2008 Jul;46(7):2402-5. doi: 10.1128/JCM.00655-08. Epub 2008 May 28.
Abstract
The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.
摘要
报道了猪微小双股RNA病毒（PBV）毒株全长基因组2片段的分子特征，以及用于检测粪便中该病毒的特异性逆转录-聚合酶链反应（RT-PCR）检测方法的开发。系统发育分析表明，所研究的猪分离株与属于基因组I的人类PBV的亲缘关系比与迄今描述的其他猪PBV更密切（同一性>85%）。对在委内瑞拉和阿根廷采集的粪便样本进行RT-PCR和聚丙烯酰胺凝胶电泳分析表明，PBV在仔猪中高频率传播。
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