de Oliveria Diêgo Madureira, Barreto George, De Andrade Deyse Valverde G, Saraceno Ezequiel, Aon-Bertolino Laura, Capani Francisco, Dos Santos El Bachá Ramon, Giraldez Lisandro Diego
Laboratório de Neuroquímica e Biologia Celular, Universidade Federal da Bahia (UFBA), Salvador, Bahia, Brazil.
Neurochem Res. 2009 Feb;34(2):215-20. doi: 10.1007/s11064-008-9749-y. Epub 2008 May 30.
Parkinson's disease (PD) is one of the most important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of Valeriana officinalis on rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells were demonstrated. The cytotoxicity, cell viability and analysis of cellular morphology were performed by MTT-tetrazole (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and phase contrast microscopy, respectively. Significant changes in the cellular morphology, and condensation of the cell body could be observed when cells were treated with 300 nM rotenone for 48 h. Three different concentrations of Valeriana officinalis extract were used (0.049, 0.098 and 0.195 mg/mL). These extracts brought about an increase of 7.0 +/- 1.3%, 14.5 +/- 1.3% and 14.5 +/- 3.2% in cell viability. Our results indicated that neuroprotector action of the Valeriana officinalis extract provides support for later studies as they help understanding this drug for the development of cytoprotective various therapies in PD.
帕金森病（PD）是全球最重要的神经退行性疾病之一。已证实缬草水提取物对鱼藤酮诱导的人神经母细胞瘤SH-SY5Y细胞凋亡具有潜在的细胞保护作用。分别通过MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法和相差显微镜检测细胞毒性、细胞活力并分析细胞形态。当用300 nM鱼藤酮处理细胞48小时时，可观察到细胞形态的显著变化以及细胞体的凝聚。使用了三种不同浓度的缬草提取物（0.049、0.098和0.195 mg/mL）。这些提取物使细胞活力分别提高了7.0±1.3%、14.5±1.3%和14.5±3.2%。我们的结果表明，缬草提取物的神经保护作用为后续研究提供了支持，因为它们有助于了解这种药物，以开发针对帕金森病的各种细胞保护疗法。
