Center of Drug Metabolism and Pharmacokinetic, China Pharmaceutical University, Nanjing 210009, China.
Phytomedicine. 2008 Nov;15(11):1016-20. doi: 10.1016/j.phymed.2008.02.021. Epub 2008 Jun 9.
The antitumor activities of six C-21 steroidal glycosides isolated from the root tuber of Cynanchum auriculatum Royle ex Wight were performed according to a microculture tetrazolium (MTT) method on human tumor cell lines SMMC-7721, MCF-7 and Hela. Of these compounds, caudatin-2,6-dideoxy-3-O-methy-β-D-cymaropyranoside and caudatin were found to be of the highest effects against human tumor cell line SMMC-7721 with IC(50) values of 13.49 and 24.95 μM, respectively. Then the in vivo assay further showed that caudatin-2,6-dideoxy-3-O-methy-β-D-cymaropyranoside and caudatin significantly inhibited the growth of transplantable H(22) tumors in mice.
从杠柳根皮中分离得到的 6 种 C-21 甾体糖苷，采用微量细胞培养噻唑蓝（MTT）比色法，对人肝癌细胞株 SMMC-7721、人乳腺癌细胞株 MCF-7 和人宫颈癌细胞株 Hela 的抗肿瘤活性进行了研究。结果表明，杠柳苷元-2,6-二去氧-3-O-甲基-β-D-吡喃毛地黄糖苷和杠柳苷元对人肝癌细胞株 SMMC-7721 的抑制活性最强，IC50 值分别为 13.49μM 和 24.95μM。体内实验进一步表明，杠柳苷元-2,6-二去氧-3-O-甲基-β-D-吡喃毛地黄糖苷和杠柳苷元能显著抑制荷瘤小鼠移植性 H（22）肿瘤的生长。
