Santos Adriana Oliveira Dos, Ueda-Nakamura Tânia, Dias Filho Benedito Prado, Veiga Junior Valdir F, Pinto Angelo C, Nakamura Celso Vataru
Programa de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Mem Inst Oswaldo Cruz. 2008 May;103(3):277-81. doi: 10.1590/s0074-02762008005000015. Epub 2008 Apr 30.
The antimicrobial activity of copaiba oils was tested against Gram-positive and Gram-negative bacteria, yeast, and dermatophytes. Oils obtained from Copaifera martii, Copaifera officinalis, and Copaifera reticulata (collected in the state of Acre) were active against Gram-positive species (Staphylococcus aureus, methicillin-resistant S. aureus, Staphylococcus epidermidis, Bacillus subtilis, and Enterococcus faecalis) with minimum inhibitory concentrations ranging from 31.3-62.5 microg/ml. The oils showed bactericidal activity, decreasing the viability of these Gram-positive bacteria within 3 h. Moderate activity was observed against dermatophyte fungi (Trichophyton rubrum and Microsporum canis). The oils showed no activity against Gram-negative bacteria and yeast. Scannning electron microscopy of S. aureus treated with resin oil from C. martii revealed lysis of the bacteria, causing cellular agglomerates. Transmission electron microscopy revealed disruption and damage to the cell wall, resulting in the release of cytoplasmic compounds, alterations in morphology, and a decrease in cell volume, indicating that copaiba oil may affect the cell wall.
对古巴香脂油针对革兰氏阳性菌、革兰氏阴性菌、酵母菌和皮肤癣菌的抗菌活性进行了测试。从马氏古柯（Copaifera martii）、药用古柯（Copaifera officinalis）和网状古柯（Copaifera reticulata，采集于阿克里州）获得的油对革兰氏阳性菌（金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌和粪肠球菌）具有活性，最低抑菌浓度范围为31.3 - 62.5微克/毫升。这些油表现出杀菌活性，在3小时内降低了这些革兰氏阳性菌的活力。观察到对皮肤癣菌（红色毛癣菌和犬小孢子菌）有中等活性。这些油对革兰氏阴性菌和酵母菌无活性。用马氏古柯的树脂油处理金黄色葡萄球菌后的扫描电子显微镜显示细菌裂解，导致细胞团聚。透射电子显微镜显示细胞壁受到破坏和损伤，导致细胞质化合物释放、形态改变和细胞体积减小，表明古巴香脂油可能影响细胞壁。
