Knyazev P G, Korobitsyn L P, Zhudina A I, Shmelkova V I, Kuznetsov O K, Dyadkova A M, Seitz I F
Neoplasma. 1976;23(5):483-94.
An infectious process was reproduced in the culture of chick embryo cells by means of DNA isolated from Rous chick sarcoma tissue (Carr-Zilber strain). This DNA preparation displays biological activity also in the culture of human embryo diploid cells (HEDC) which is manifested in: 1. discontinuous synthesis of avian oncovirus group-specific antigen; 2. enhancement of proliferative activity and morphological transformation of human cells; 3. continuous presence of virus-specific sequences as revealed by DNA/RNA hybridization. Producing complete oncornavirus by means of DNA isolated from Rous chick sarcoma in HEDC was unsuccessful. DNA preparation from gs negative chick embryo cells shows no infectious activity in HEDC culture.
通过从劳氏鸡肉瘤组织（卡尔 - 齐尔伯株）分离的DNA，在鸡胚细胞培养物中重现了感染过程。这种DNA制剂在人胚二倍体细胞（HEDC）培养物中也表现出生物活性，具体表现为：1. 禽肿瘤病毒群特异性抗原的间断合成；2. 人细胞增殖活性增强和形态转化；3. DNA/RNA杂交显示病毒特异性序列持续存在。利用从劳氏鸡肉瘤分离的DNA在HEDC中产生完整的肿瘤病毒未获成功。来自gs阴性鸡胚细胞的DNA制剂在HEDC培养物中无感染活性。
