尿液中扁桃酸和苯乙醛酸的毛细管气相色谱法。
Capillary gas chromatographic method for mandelic and phenylglyoxylic acids in urine.
作者信息
Dills R L, Wu R L, Checkoway H, Kalman D A
机构信息
Department of Environmental Health, School of Public Health and Community Medicine, University of Washington, Seattle 98195.
出版信息
Int Arch Occup Environ Health. 1991;62(8):603-6. doi: 10.1007/BF00381115.
In support of an occupational investigation of styrene exposure, a capillary gas chromatographic method was developed for the quantitation of the styrene metabolites mandelic and phenylglyoxylic acids. The method was based on that of Guillemin and Bauer, in which phenylglyoxylic acid was converted to mandelic acid by reduction before instrumental analysis. The earlier method had to be modified for use with capillary columns; the resulting method was sensitive, selective and reproducible. The detection limit was approximately 0.001 mg/ml urine. Approximately less than 5% relative precision was achieved in the range of 0.05-2 mg/ml urine. Mandelic acid was resolved from other components of urine and from by-products of derivatization.
为支持一项关于苯乙烯暴露的职业调查，开发了一种毛细管气相色谱法用于定量分析苯乙烯代谢产物扁桃酸和苯乙醛酸。该方法基于吉耶曼和鲍尔的方法，在仪器分析前，苯乙醛酸通过还原转化为扁桃酸。早期方法必须进行修改以用于毛细管柱；所得方法灵敏、选择性好且可重现。检测限约为0.001mg/ml尿液。在0.05 - 2mg/ml尿液范围内，相对精密度约低于5%。扁桃酸与尿液中的其他成分以及衍生化副产物得以分离。
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