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将鸡蛋蛋清作为培养三维多细胞肿瘤球体的新型平台的特性研究

Characterization of Hen's Egg White To Use It as a Novel Platform To Culture Three-Dimensional Multicellular Tumor Spheroids.

作者信息

Balaji Perumalsamy, Murugadas Anbazhagan, Ramkumar Arunachalam, Thirumurugan Ramasamy, Shanmugaapriya Sellathamby, Akbarsha Mohammad Abdulkader

机构信息

Department of Biomedical Science, Bharathidasan University, Tiruchirapalli 620024, India.

National Centre for Alternatives to Animal Experiments, Bharathidasan University, Tiruchirappalli 620 024, India.

出版信息

ACS Omega. 2020 Aug 3;5(31):19760-19770. doi: 10.1021/acsomega.0c02508. eCollection 2020 Aug 11.

DOI:10.1021/acsomega.0c02508
PMID:32803071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7424746/
Abstract

We are standardizing protocols to develop egg white (EW) as a cost-effective platform for culture of three-dimensional (3-D) multicellular tumor spheroids for application in understanding tumor microenvironments and drug screening. In this article, we describe several physical and physiological characteristics of EW to use it as 3-D cell culture platform. Field emission scanning electron microscopy revealed the presence of different microstructures. Hydrodynamic size distribution data indicated nano- and micron-sized particles. Rheological measurements revealed the viscosity and viscoelastic behavior appropriate for maintaining cell viability and supporting 3-D cell growth under high-sheer conditions. It was found that thereis no autofluorescence, a requirement for imparting transparency and for microscopic observations of the spheroids. The EW facilitated the development of 3-D tumor spheroids, with an emphasis of difference in cell proliferation and intercellular cytoskeletal organization between two-dimensional and 3-D spheroid cultures. Put together, EW proves to be a cost-affordable and simple platform for 3-D culture of tumor spheroids.

摘要

我们正在规范相关方案，以开发蛋清（EW）作为一种经济高效的平台，用于三维（3-D）多细胞肿瘤球体的培养，以应用于肿瘤微环境的研究和药物筛选。在本文中，我们描述了EW作为3-D细胞培养平台的几种物理和生理特性。场发射扫描电子显微镜揭示了不同微观结构的存在。流体动力学尺寸分布数据表明存在纳米级和微米级颗粒。流变学测量显示其粘度和粘弹性行为适合在高剪切条件下维持细胞活力并支持3-D细胞生长。研究发现，EW不存在自发荧光，这对于赋予球体透明度以及进行显微镜观察是必需的。EW促进了3-D肿瘤球体的形成，重点在于二维和3-D球体培养之间细胞增殖和细胞间细胞骨架组织的差异。综上所述，EW被证明是一种经济实惠且简单的肿瘤球体3-D培养平台。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96f9/7424746/5cdde98c24d7/ao0c02508_0015.jpg
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Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.胶质母细胞瘤干细胞在肿瘤血管周龛中受白细胞介素-8 信号的调节。
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