Chan Eugene W L, Yousaf Muhammad N
Department of Chemistry and the Carolina Center for Genome Science, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
Mol Biosyst. 2008 Jul;4(7):746-53. doi: 10.1039/b801394b. Epub 2008 May 20.
We report a combined photochemical and electrochemical method to pattern ligands and cells in complex geometries and gradients on inert surfaces. This work demonstrates: (1) the control of density of immobilized ligands within overlapping photopatterns, and (2) the attached cell culture patterned onto ligand defined gradients for studies of directional cell polarity. Our approach is based on the photochemical activation of benzoquinonealkanethiols. Immobilization of aminooxy terminated ligands in selected region of the quinone monolayer resulted in patterns on the surface. This approach is unique in that the extent of photochemical deprotection, as well as ligand immobilization can be monitored and quantified by cyclic voltammetry in situ. Furthermore, complex photochemical patterns of single or multiple ligands can be routinely generated using photolithographic masks. Finally, this methodology is completely compatible with attached cell culture and we show how the subtle interplay between cell-cell interactions and underlying peptide gradient influences cell polarization. The combined use of photochemistry, electrochemistry and well defined surface chemistry provides molecular level control of patterned ligands and gradients on surfaces.
我们报道了一种结合光化学和电化学的方法，用于在惰性表面上以复杂的几何形状和梯度对配体和细胞进行图案化。这项工作证明了：（1）在重叠的光图案中控制固定化配体的密度，以及（2）将附着的细胞培养物图案化到配体定义的梯度上，用于研究细胞定向极性。我们的方法基于苯醌烷硫醇的光化学活化。在醌单层的选定区域固定氨基氧基末端的配体导致表面上形成图案。这种方法的独特之处在于，光化学脱保护的程度以及配体固定化可以通过循环伏安法原位监测和量化。此外，使用光刻掩模可以常规生成单个或多个配体的复杂光化学图案。最后，这种方法与附着细胞培养完全兼容，我们展示了细胞间相互作用与潜在肽梯度之间的微妙相互作用如何影响细胞极化。光化学、电化学和明确的表面化学的结合使用提供了对表面上图案化配体和梯度的分子水平控制。
