Department of Biomedical Engineering, University of Wisconsin, 1550 Engineering Drive, Engineering Centers Building, Madison, WI 53706, USA.
Chembiochem. 2012 Aug 13;13(12):1717-24. doi: 10.1002/cbic.201200226. Epub 2012 Jul 17.
Self-assembled monolayers (SAMs) of alkanethiolates on gold can be used to carefully probe immobilized biomolecule interactions with cell-surface receptors. However, due to a lack of experimental throughput associated with labor-intensive production, specialized fabrication apparatus, and other practical challenges, alkanethiolate SAMs have not had widespread use by biological researchers. In this Minireview, we investigate a range of techniques that could enhance the throughput of SAM-based approaches by patterning substrates with arrays of different conditions. Here we highlight microfluidic, photochemical, localized removal, and backfilling techniques to locally pattern SAM substrates with biomolecules and also describe how these approaches have been applied in SAM-based screening systems. Furthermore we provide perspectives on several crucial barriers that need to be overcome to enable widespread use of SAM chemistry in biological applications.
自组装单分子层（SAMs）可以用于仔细探测固定化生物分子与细胞表面受体的相互作用。然而，由于缺乏与劳动密集型生产、专用制造仪器和其他实际挑战相关的实验通量，烷硫醇 SAM 并没有被生物研究人员广泛使用。在这篇综述中，我们研究了一系列可以通过在不同条件的阵列上对底物进行图案化来提高基于 SAM 的方法通量的技术。在这里，我们重点介绍微流控、光化学、局部去除和回流技术，这些技术可以将生物分子局部图案化到 SAM 底物上，并描述这些方法在基于 SAM 的筛选系统中的应用。此外，我们还提供了一些观点，指出了在 SAM 化学在生物应用中得到广泛应用之前需要克服的几个关键障碍。
