Yi L, Lin J Y, Gao Y, Feng Z J, Wang D X
Department of Neurology, Beijieng Friendship Hospital, Capital Medical University, 95 Yong'an Street, Xuan Wu District, Beijing, 100050 PR China.
Acta Virol. 2008;52(2):99-106.
The association of atherosclerosis (AS) and Human cytomegalovirus (HCMV) infection was studied. AS plays an important role in the brain stroke and HCMV infection is supposed to be involved in the process of atherosclerotic formation. The presence of HCMV DNA and antigens was examined in the internal carotid arteries collected from 35 patients with ischemic stroke and from 20 patients from the control population. All patients belonged to the ethnic Han population in China. Three methods, immunohistochemistry (IHC), hybridization in situ (HIS), and PCR were used to detect the HCMV immediate early (IE) and late (L) antigens as well as viral DNA in vessel walls. Levels of HCMV IE gene/protein were significantly higher in the stroke group than in control group detected by the three methods (IHC 34.3% vs. 10.0%; HIS 40.0% vs. 10.0; PCR 60.0% vs. 30.0%). However, there was no significant difference in the levels of HCMV L gene/protein between these two groups of patients (IHC 11.4% vs. 5.0%; HIS 11.4% vs. 10.0%; PCR 20.0% vs. 20.0%). We concluded that the presence of HCMV IE antigen and HCMV DNA in the vessel wall was associated with the pathological process of AS formation.
研究了动脉粥样硬化（AS）与人类巨细胞病毒（HCMV）感染之间的关联。AS在脑卒风中起重要作用，并且HCMV感染被认为参与动脉粥样硬化形成过程。检测了从35例缺血性脑卒中患者和20例对照人群中采集的颈内动脉中HCMV DNA和抗原的存在情况。所有患者均为中国汉族人群。采用免疫组织化学（IHC）、原位杂交（HIS）和聚合酶链反应（PCR）三种方法检测血管壁中HCMV即刻早期（IE）和晚期（L）抗原以及病毒DNA。通过三种方法检测，脑卒中组中HCMV IE基因/蛋白水平显著高于对照组（IHC：34.3%对10.0%；HIS：40.0%对10.0；PCR：60.0%对30.0%）。然而，两组患者之间HCMV L基因/蛋白水平无显著差异（IHC：11.4%对5.0%；HIS：11.4%对10.0%；PCR：20.0%对20.0%）。我们得出结论，血管壁中HCMV IE抗原和HCMV DNA的存在与AS形成的病理过程相关。
