Rotimi Vincent O, Jamal Wafaa, Pal Tibor, Sovenned Agnes, Albert M John
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Department of Microbiology & Immunology, Pecs University, Pecs, Hungary.
J Med Microbiol. 2008 Jul;57(Pt 7):881-886. doi: 10.1099/jmm.0.47509-0.
Cephalosporins are major antimicrobials used to treat serious Salmonella infections. However, their effectiveness is being compromised by the emergence of extended-spectrum beta-lactamases (ESBLs). The genetic determinants encoding ESBL in Salmonella spp. isolated from patients in Kuwait and United Arab Emirates (UAE) were studied over a 2 year period. Out of a total of 407 isolates, 116 isolates possessed the resistance phenotypes consistent with possible ESBL production. Of these, 69 (59.5 %) were ESBL positive. PCR and sequencing were used to determine the genetic determinant(s) responsible for ESBL phenotypes. A total of 14 (12.1 %) and 29 (24.6 %) isolates were CTX-M-15 ESBL producers and TEM producers, respectively. Ten CTX-M-15 producers carried the insertion sequence ISEcpI gene. PFGE analysis revealed identical profiles in 4 of the 13 Kuwaiti strains. This study reports the presence of the bla(CTX-M-15) gene in Salmonella spp. and Salmonella enterica serotype Typhi from Kuwait and UAE for what is believed to be the first time. This is of great concern as the gene is also found in association with the ISEcpI gene, which may easily facilitate its spread. These isolates originated mostly from non-Kuwaiti Arabs rather than from people of Asian origin.
头孢菌素是用于治疗严重沙门氏菌感染的主要抗菌药物。然而，超广谱β-内酰胺酶（ESBLs）的出现正使其有效性受到损害。在两年时间里，对从科威特和阿拉伯联合酋长国（阿联酋）患者中分离出的沙门氏菌属中编码ESBL的遗传决定因素进行了研究。在总共407株分离株中，有116株具有与可能产生ESBL一致的耐药表型。其中，69株（59.5%）为ESBL阳性。采用聚合酶链反应（PCR）和测序来确定导致ESBL表型的遗传决定因素。分别有14株（12.1%）和29株（24.6%）分离株是CTX-M-15型ESBL生产者和TEM型生产者。10株CTX-M-15型生产者携带插入序列ISEcpI基因。脉冲场凝胶电泳（PFGE）分析显示，13株科威特菌株中有4株具有相同的图谱。本研究首次报道了在来自科威特和阿联酋的沙门氏菌属和伤寒沙门氏菌中存在bla(CTX-M-15)基因。这令人高度关注，因为该基因还与ISEcpI基因相关联，这可能很容易促进其传播。这些分离株大多来自非科威特阿拉伯人，而非亚洲血统的人。
