使用SeqMap网络资源对基因治疗研究中的病毒整合位点进行自动化分析。
Automated analysis of viral integration sites in gene therapy research using the SeqMap web resource.
作者信息
Peters B, Dirscherl S, Dantzer J, Nowacki J, Cross S, Li X, Cornetta K, Dinauer M C, Mooney S D
机构信息
Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN, USA.
出版信息
Gene Ther. 2008 Sep;15(18):1294-8. doi: 10.1038/gt.2008.99. Epub 2008 Jun 26.
Abstract
Research in gene therapy involving genome-integrating vectors now often includes analysis of vector integration sites across the genome using methods such as ligation-mediated PCR (LM-PCR) or linear amplification-mediated PCR (LAM-PCR). To help researchers analyze these sites and the functions of nearby genes, we have developed SeqMap (http://seqmap.compbio.iupui.edu/) a secure, web-based comprehensive vector integration site management tool that automatically analyzes and annotates large numbers of vector integration sites derived from LM-PCR experiments in human and model organisms upon a common genome database. We believe the use of this resource will enable better reproducibility and understanding of this important data.
摘要
涉及基因组整合载体的基因治疗研究现在通常包括使用连接介导的PCR（LM-PCR）或线性扩增介导的PCR（LAM-PCR）等方法分析全基因组中的载体整合位点。为了帮助研究人员分析这些位点以及附近基因的功能，我们开发了SeqMap（http://seqmap.compbio.iupui.edu/），这是一个基于网络的安全、全面的载体整合位点管理工具，它能在一个通用基因组数据库上自动分析和注释来自人类和模式生物LM-PCR实验的大量载体整合位点。我们相信使用这一资源将能更好地重现和理解这些重要数据。
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