Cai Man, Zhi Xiao-Yang, Tang Shu-Kun, Zhang Yu-Qin, Xu Li-Hua, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1556-60. doi: 10.1099/ijs.0.65513-0.
An actinomycete strain, designated YIM 91355(T), was isolated from a soil sample collected from a hypersaline soil in Xinjiang Province, China, and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium of this organism was well developed but not fragmented and, at maturity, formed short chains of spores. Substrate mycelium was branched with non-fragmenting hyphae and single, oval to round spores were formed, borne on sporophores. Strain YIM 91355(T) contained meso-diaminopimelic acid as the diagnostic amino acid. The purified cell-wall hydrolysate contained galactose. The predominant menaquinones were MK-10(H(8)) (43.2 %), MK-10(H(6)) (17.7 %) and MK-11(H(8)) (10.4 %). Phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylcholine, phosphatidylinositol and an unidentified phospholipid. The major fatty acids were i-C(16 : 0), ai-C(17 : 0), 10-methyl C(17 : 0) and 10-methyl C(18 : 0). The G+C content of the genomic DNA was 72.1 mol%. Phylogenetic analysis indicated that the isolate belongs to the genus Streptomonospora of the family Nocardiopsaceae. Based on phenotypic and genotypic data, it is concluded that strain YIM 91355(T) represents a novel species of the genus Streptomonospora, for which the name Streptomonospora halophila sp. nov. (type strain YIM 91355(T) =DSM 45075(T) =KCTC 19236(T)) is proposed.
从中国新疆高盐土壤采集的一份土壤样品中分离出一株放线菌，编号为YIM 91355(T)，并采用多相分类法对其分类地位进行了研究。该菌株在大多数测试培养基上生长良好，不产生可扩散色素。该菌的气生菌丝发育良好但不分支，成熟时形成短链孢子。基内菌丝有分支，菌丝不分裂，形成单个、椭圆形至圆形的孢子，着生于孢梗上。菌株YIM 91355(T)含内消旋二氨基庚二酸作为特征性氨基酸。纯化的细胞壁水解产物含有半乳糖。主要的甲基萘醌为MK-10(H(8))（43.2%）、MK-10(H(6))（17.7%）和MK-11(H(8))（10.4%）。磷脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰胆碱、磷脂酰肌醇和一种未鉴定的磷脂。主要脂肪酸为异-C(16 : 0)、anteiso-C(17 : 0)、10-甲基-C(17 : 0)和10-甲基-C(18 : 0)。基因组DNA的G+C含量为72.1 mol%。系统发育分析表明，该分离株属于诺卡氏菌科链单孢菌属。基于表型和基因型数据，得出结论：菌株YIM 91355(T)代表链单孢菌属的一个新物种，为此提出新名称嗜盐链单孢菌（Streptomonospora halophila sp. nov.）（模式菌株YIM 91355(T) =DSM 45075(T) =KCTC 19236(T)）。
