Yoon Jung-Hoon, Kang So-Jung, Jung Yong-Taek, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1603-7. doi: 10.1099/ijs.0.65659-0.
A Gram-negative, non-motile, rod-shaped bacterial strain, SMK-108(T), was isolated from a tidal flat of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic investigation. Strain SMK-108(T) grew optimally at pH 7.0-8.0 and at 30 degrees C. It contained MK-6 as the predominant menaquinone. The major fatty acids were iso-C(17 : 0) 3-OH, iso-C(15 : 1) and iso-C(15 : 0). The DNA G+C content was 41.1 mol%. Comparative 16S rRNA gene sequence analysis showed that strain SMK-108(T) was related most closely to members of the genus Muricauda, exhibiting 96.6-98.8 % sequence similarity to the type strains of recognized Muricauda species. Strain SMK-108(T) was distinguishable from recognized Muricauda species on the basis of differential phenotypic characteristics, levels of DNA-DNA relatedness and phylogenetic distinctiveness. This organism is thus considered to represent a novel species of the genus Muricauda, for which the name Muricauda lutimaris sp. nov. is proposed. The type strain is SMK-108(T) (=KCTC 22173(T) =CCUG 55324(T)).
从韩国黄海潮间带分离出一株革兰氏阴性、无运动性、杆状的细菌菌株SMK-108(T)，并对其进行了多相分类学研究。菌株SMK-108(T)在pH 7.0 - 8.0和30℃条件下生长最佳。它以MK-6作为主要的甲基萘醌。主要脂肪酸为异-C(17 : 0) 3-OH、异-C(15 : 1)和异-C(15 : 0)。DNA G+C含量为41.1 mol%。比较16S rRNA基因序列分析表明，菌株SMK-108(T)与Muricauda属的成员关系最为密切，与已确认的Muricauda属物种的模式菌株的序列相似性为96.6 - 98.8%。基于不同的表型特征、DNA-DNA相关性水平和系统发育独特性，菌株SMK-108(T)可与已确认的Muricauda属物种区分开来。因此，该菌株被认为代表了Muricauda属的一个新物种，为此提出了新名称Muricauda lutimaris sp. nov.。模式菌株为SMK-108(T) (=KCTC 22173(T) =CCUG 55324(T))。
