Department of Cellular Biology, Biological Sciences Institute, University of Brasília, Brasília, DF, Brazil.
Department of Civil and Environmental Engineering, University of Brasília, Brasília, DF, Brazil.
Braz J Microbiol. 2021 Mar;52(1):325-333. doi: 10.1007/s42770-020-00400-3. Epub 2020 Nov 5.
Strain K001 was isolated from a cyanobacterial culture derived from Abrolhos, a reef bank microbial mat (South Atlantic Ocean-Brazil). Cells of K001 are Gram stain-negative, catalase and oxidase-positive, non-motile, rod-shaped, and with or without appendages. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K001 belongs to the genus Muricauda. The highest strain K001 16S rRNA gene identity, ANI, and dDDH, respectively, are with M. aquimarina (98.90%, 79.23, 21.60%), M. ruestringensis (98.20%, 80.82, 23.40%), and M. lutimaris (97.86%, 79.23, 22.70%). The strain grows at 15-37 °C and between 0.5 and 10% NaCl. The major fatty acids of strain K001 are iso-C, iso-C G, iso-C 3-OH, and summed feature 3 (C ω6c and/or C ω7c). The polar lipids are represented by phosphatidylethanolamine, three unidentified aminolipids, and three unidentified polar lipids. The major respiratory quinone is MK-6. The G+C content of the DNA of strain K001 is 41.62 mol%. Based on polyphasic analysis of strain K001, it was identified as a novel representative of the genus Muricauda and was named Muricauda brasiliensis sp. nov. The type strain is K001 (=CBMAI 2315 = CBAS 752).
K001 菌株从 Abrolhos 的蓝细菌培养物中分离得到，Abrolhos 是一个珊瑚礁微生物垫（南大西洋-巴西）。K001 细胞革兰氏染色阴性，过氧化氢酶和氧化酶阳性，不运动，呈杆状，有或没有附属物。基于 16S rRNA 基因序列的系统发育分析表明，菌株 K001 属于 Muricauda 属。K001 菌株的 16S rRNA 基因序列与 Muricauda aquimarina（98.90%、79.23%、21.60%）、Muricauda ruestringensis（98.20%、80.82%、23.40%）和 Muricauda lutimaris（97.86%、79.23%、22.70%）的最高同源性分别为 98.90%、79.23%和 21.60%。该菌株在 15-37°C 和 0.5-10%NaCl 下生长。K001 菌株的主要脂肪酸为 iso-C、iso-C G、iso-C 3-OH 和特征脂肪酸 3（C ω6c 和/或 C ω7c）。极性脂包括磷脂酰乙醇胺、三种未鉴定的胺脂和三种未鉴定的极性脂。主要呼吸醌为 MK-6。K001 菌株的 DNA G+C 含量为 41.62mol%。基于 K001 菌株的多相分析，将其鉴定为 Muricauda 属的一个新代表种，并命名为 Muricauda brasiliensis sp. nov. 模式菌株为 K001（=CBMAI 2315=CBAS 752）。
