Fleming Christine P, Ripplinger Crystal M, Webb Bryan, Efimov Igor R, Rollins Andrew M
Case Western Reserve University, Biomedical Engineering Department, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.
J Biomed Opt. 2008 May-Jun;13(3):030505. doi: 10.1117/1.2937470.
Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged.
心脏组织微观结构的异质性是心律失常产生和维持的潜在机制。纤维方向的异常变化会增加心律失常的可能性。我们提出光学相干断层扫描(OCT)作为一种对完整离体心脏标本中的肌纤维进行成像的方法。使用显微镜集成OCT系统从兔右心室游离壁(RVFW)收集三维(3-D)图像集。开发了一种用于在平行于壁表面的平面中进行纤维方向量化的自动算法。该算法通过与手动测量结果进行比较得到验证。从OCT图像中量化平行于壁表面的平面中的纤维方向可用于帮助理解所成像的特定样本的传导系统。