野葛水提取物在人包皮成纤维细胞系中抑制肠道病毒71的细胞毒性。
A water extract of Pueraria lobata inhibited cytotoxicity of enterovirus 71 in a human foreskin fibroblast cell line.
作者信息
Su Fu-Min, Chang Jung-San, Wang Kuo-Chih, Tsai Jih-Jin, Chiang Lien-Chai
机构信息
Department of Pharmacy, Kaohsiung Medical University Hospital, Kaohsiung Medical University, and Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
Kaohsiung J Med Sci. 2008 Oct;24(10):523-30. doi: 10.1016/S1607-551X(09)70011-7.
Enterovirus 71 (EV71) can cause brain encephalitis and mortality. However, effective vaccines or chemotherapeutic agents are not yet available. We tested the hypothesis that Pueraria lobata could inhibit the cytotoxic effect of EV71 in a human foreskin fibroblast cell line by the XTT method. Our results showed that the water extract of P. lobata could inhibit cytopathy induced by EV71 when given before (p < 0.0001), simultaneously with (p < 0.0001), or after viral infection (p < 0.0001). Water extract of P. lobata was effective and its minimal concentration that inhibited 50% of the cytopathic effect (IC50) was 0.028 microg/mL. P. lobata was also safe with a selectivity index greater than 107,000. Water extract of P. lobata appeared to inhibit viral attachment (p < 0.0001) and penetration (p < 0.0001). The anti-EV71 activity of the water extract of P. lobata was not mediated by interferons. In conclusion, the water extract of P. lobata was effective in the management of the disease induced by EV71 infection.
肠道病毒71型（EV71）可引发脑脑炎并导致死亡。然而，目前尚无有效的疫苗或化疗药物。我们通过XTT法检验了葛根能抑制EV71对人包皮成纤维细胞系细胞毒性作用的假说。我们的结果显示，葛根水提取物在病毒感染前（p < 0.0001）、同时（p < 0.0001）或感染后（p < 0.0001）给予时，均可抑制EV71诱导的细胞病变。葛根水提取物有效，其抑制50%细胞病变效应（IC50）的最小浓度为0.028微克/毫升。葛根的选择性指数大于107,000，也是安全的。葛根水提取物似乎能抑制病毒附着（p < 0.0001）和穿透（p < 0.0001）。葛根水提取物的抗EV71活性并非由干扰素介导。总之，葛根水提取物对EV71感染所致疾病的治疗有效。
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