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重症新生儿和儿童脓毒症患者中性粒细胞和单核细胞CD64及CD163表达：荧光强度及计算指标比较

Neutrophil and monocyte CD64 and CD163 expression in critically ill neonates and children with sepsis: comparison of fluorescence intensities and calculated indexes.

作者信息

Groselj-Grenc Mojca, Ihan Alojz, Derganc Metka

机构信息

Department of Paediatric Surgery and Intensive Care, University Medical Center, Zaloska 7, 1525 Ljubljana, Slovenia.

出版信息

Mediators Inflamm. 2008;2008:202646. doi: 10.1155/2008/202646.

DOI:10.1155/2008/202646
PMID:18604302
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2442385/
Abstract

OBJECTIVE

To evaluate the expression of CD64 and CD163 on neutrophils and monocytes in SIRS with/without sepsis and to compare the diagnostic accuracy of CD64 and CD163 molecules expression determined as (1) mean fluorescence intensities (MFI) of CD64 and CD163; and (2) the ratio (index) of linearized MFI to the fluorescence signal of standardized beads.

PATIENTS AND METHODS

Fifty-six critically ill neonates and children with systemic inflammatory response syndrome (SIRS) and suspected sepsis, classified into two groups: SIRS with sepsis (n = 29) and SIRS without sepsis (n = 27).

RESULTS

CD64 and CD163 MFI measured on neutrophils and monocytes were elevated in patients with SIRS with sepsis. Diagnostic accuracy of indexes was equal to diagnostic accuracy of MFI for CD64 on neutrophils (0.833 versus 0.854 for day 0 and 0.975 versus 0.983 for day 1) and monocytes (0.811 versus 0.865 for day 0 and 0.825 versus 0.858 for day 1), and CD163 on neutrophils (0.595 versus 0.655 for day 0 and 0.677 versus 0.750 for day 1), but not for CD163 on monocytes.

CONCLUSION

CD64 MFI, CD163 MFI, CD64 indexes for neutrophils and monocytes, and CD163 index for neutrophils can all be used for discrimination of SIRS and sepsis in critically ill neonates and children. CD64 index for neutrophils, however, is superior to all other markers.

摘要

目的

评估伴或不伴脓毒症的全身炎症反应综合征（SIRS）患者中性粒细胞和单核细胞上CD64和CD163的表达，并比较以（1）CD64和CD163的平均荧光强度（MFI）；以及（2）线性化MFI与标准化微珠荧光信号的比值（指数）所测定的CD64和CD163分子表达的诊断准确性。

患者与方法

56例患有全身炎症反应综合征（SIRS）且疑似脓毒症的危重新生儿和儿童，分为两组：伴有脓毒症的SIRS（n = 29）和不伴有脓毒症的SIRS（n = 27）。

结果

伴有脓毒症的SIRS患者中性粒细胞和单核细胞上测得的CD64和CD163 MFI升高。指数的诊断准确性与中性粒细胞（第0天为0.833对0.854，第1天为0.975对0.983）和单核细胞（第0天为0.811对0.865，第1天为0.825对0.858）上CD64的MFI诊断准确性相同，以及与中性粒细胞上CD163的MFI诊断准确性相同（第0天为0.595对0.655，第1天为0.677对0.750），但与单核细胞上CD163的MFI诊断准确性不同。

结论

CD64 MFI、CD163 MFI、中性粒细胞和单核细胞的CD64指数以及中性粒细胞的CD163指数均可用于区分危重新生儿和儿童的SIRS和脓毒症。然而，中性粒细胞的CD64指数优于所有其他标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/7d86549a1a2c/MI2008-202646.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/0a1dc7585137/MI2008-202646.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/efc8e36a7762/MI2008-202646.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/7d86549a1a2c/MI2008-202646.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/0a1dc7585137/MI2008-202646.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/efc8e36a7762/MI2008-202646.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ad5/2442385/7d86549a1a2c/MI2008-202646.003.jpg

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