Ciuffetti G, Mercuri M, Mannarino E, Ott C, Lombardini R, Pasqualini L, Lupattelli G, Santambrogio L
2nd Department of Internal Medicine, University of Perugia, Italy.
Int Angiol. 1991 Apr-Jun;10(2):81-7.
Malondialdehyde (MDA) plasma concentrations (marker of lipid free radical peroxidation) and the unfractionated leucocyte and erythrocyte filterability rates (through 5 micron Nuclepore filters in a positive pressure filtration system) were determined during a partial ischaemia induced by two consecutive treadmill tests in 64 stage II peripheral vascular disease (PVD) patients and in 32 matched controls. Transcutaneous oxygen pressure (tcPO2) was monitored constantly throughout the walking tests and recovery periods. In the PVD patients impairments in the flow properties of leucocytes and erythrocytes were associated with increases in MDA plasma concentrations which correlated significantly with a lengthening of the tcPO2 half recovery times--index of the worsening of ischaemia. Patients could be divided into two sub-groups on the basis of these impairments and their half recovery times, suggesting this experimental model may provide a means to identify those stage II PVD patients most likely to develop critical limb ischaemia.
在64例II期外周血管疾病（PVD）患者和32例匹配的对照组中，通过连续两次跑步机测试诱导局部缺血期间，测定了丙二醛（MDA）血浆浓度（脂质自由基过氧化的标志物）以及未分级白细胞和红细胞的过滤率（在正压过滤系统中通过5微米核孔滤膜）。在整个步行测试和恢复期间持续监测经皮氧分压（tcPO2）。在PVD患者中，白细胞和红细胞流动特性的损害与MDA血浆浓度的增加相关，MDA血浆浓度的增加与tcPO2半恢复时间的延长显著相关，tcPO2半恢复时间是缺血恶化的指标。根据这些损害及其半恢复时间，患者可分为两个亚组，这表明该实验模型可能提供一种方法来识别最有可能发展为严重肢体缺血的II期PVD患者。
